SCT281

BioTracker^™ Blue Live Cell Cycle Assay Solution

Human

• Synonym(s): cell cycle dye, cell cycle indicator, cell cycle probe, live cell probe
• UNSPSC Code: 41106514
• NACRES: NA.47

Properties

• product name: BioTracker^™ Blue Live Cell Cycle Assay Solution,
• biological source: human
• Quality Level: 100
• packaging: vial of 50 tests
• manufacturer/tradename: Millipore
• technique(s): cell culture | stem cell: suitable; flow cytometry: suitable
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

Propidium iodide (PI) is often used for cell cycle analysis by flow cytometry. Compared to this analysis method, the BioTracker^™ Far Red Live Cell Cycle Assay Solution does not require cell fixation or permeabilization, and is permeable in live cells. Use 5 uL of probe solution in 500 uL for each sample. Vial contains enough for 50 tests.IMPORTANT APPLICATION NOTES:*Wash cells thoroughly to remove trypsin. Residual trypsin can confound analysis of flow cytometry populations.*Adjust gates after cells are stained with probe SPECTRAL PROPERTIESExcitation: 405 - 407 nmEmission: 450/50 BPUse violet laser for excitation.

Features and Benefits

Blue fluorescence probe for analysis of the cell cycle by flow cytometry.

Target description

The control mechanism of the cell cycle is closely linked to cell proliferation. The cell cycle includes cell division, in which one cell produces two daughter cells. The cell cycle can be assessed by detecting cells stained for DNA using flow cytometry, followed by analysis of the proportion of stained nuclei in cell cycle phase. The cell cycle is classified into two major phases: interphase and the mitotic (M) phase. Interphase is composed of G1 phase (preparation for DNA synthesis which occurs prior to cell division), S phase (DNA synthesis and replication), and G2 phase (chromosome duplication). M phase involves mitosis and cytokinesis. Cell cycle progression is regulated by cyclins and cyclin-dependent kinases. To determine the action of drugs such as anticancer therapeutics, it is essential to analyze the mechanism of cell-cycle checkpoint.

Storage and Stability

Store BioTracker^™ Blue Live Cell Cycle Assay Solution at -20 °C, protected from light.

Other Notes

Live cell probe for fluorescent cell cycle analysis. Unlike propidium iodide (PI), this probe is membrane permeable and highly specific for DNA, which makes it possible to analyze the cell cycle by simply adding the reagent to a cell suspension without any fixation, permeabilization, or other sample prep.

Legal Information

BioTracker is a trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Pictograms: GHS05,GHS09
• Signal Word: Danger
• Hazard Statements: H318,H411
• Precautionary Statements: P273 - P280 - P305 + P351 + P338 - P391 - P501
• Hazard Classifications: Aquatic Chronic 2 - Eye Dam. 1
• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable