SCM139
EZ-LiFT Stem Cell Passaging Reagent
Enzyme-free pluripotent stem cell dissociation solution, 100mL
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About This Item
UNSPSC Code:
12352207
eCl@ss:
32160801
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form
liquid
technique(s)
cell culture | stem cell: suitable
Related Categories
General description
A common problem associated with the routine culture of human pluripotent stem cells, including ES and iPS cells, is the unwanted spontaneous differentiation of cells. Without manual intervention, differentiated cells proliferate quickly and eventually diminish the overall quality of the pluripotent stem cell culture. Manual removal of differentiated cells is laborious and time consuming, making it difficult to process multiple hES/iPS cell lines simultaneously. Moreover, the risk for contamination is present each time the culture is subjected to this clean-up process.
EZ-LiFT Stem Cell Passaging Reagent is a proprietary enzyme-free and chemically defined stem cell dissociation reagent that selectively passages only undifferentiated pluripotent stem cells. The reagent eliminates the need for manual removal of differentiated cells and produces high cell viability without the need for ROCKi. By nature of its selectivity for undifferentiated pluripotent stem cells, EZ-LiFT can be used to rescue highly differentiated hES/iPS cultures, thus enabling the recovery of precious samples and time.
EZ-LiFT does not suffer from the drawbacks of other enzyme-based dissociation reagents such as Dispase, Collagenase IV and Accutase. The disadvantages of enzyme-based reagents are that they still require manual removal of differentiated cells, suffer from lot-to-lot variability and may carry the risk for karyotypic abnormalities.
Features and Benefits
• Enzyme-free and chemically defined formulation: Produces consistent results.
• Enriches for pluripotent stem cell populations: No manual clean-up required.
• Rescues highly differentiated hES/iPS culture: Saves precious samples and time.
• Gentle on cells with high cell survival rates: ROCKi is optional, but not required.
EZ-LiFT Stem Cell Passaging Reagent is a proprietary enzyme-free and chemically defined stem cell dissociation reagent that selectively passages only undifferentiated pluripotent stem cells. The reagent eliminates the need for manual removal of differentiated cells and produces high cell viability without the need for ROCKi. By nature of its selectivity for undifferentiated pluripotent stem cells, EZ-LiFT can be used to rescue highly differentiated hES/iPS cultures, thus enabling the recovery of precious samples and time.
EZ-LiFT does not suffer from the drawbacks of other enzyme-based dissociation reagents such as Dispase, Collagenase IV and Accutase. The disadvantages of enzyme-based reagents are that they still require manual removal of differentiated cells, suffer from lot-to-lot variability and may carry the risk for karyotypic abnormalities.
Features and Benefits
• Enzyme-free and chemically defined formulation: Produces consistent results.
• Enriches for pluripotent stem cell populations: No manual clean-up required.
• Rescues highly differentiated hES/iPS culture: Saves precious samples and time.
• Gentle on cells with high cell survival rates: ROCKi is optional, but not required.
Application
Research Category
Cell Culture
Stem Cell Research
Cell Culture
Stem Cell Research
Quality
pH: 7.2-7.4
Sterility Tested: Pass
Osmolarity: 340-370 mOsm
Sterility Tested: Pass
Osmolarity: 340-370 mOsm
Storage and Stability
EZ-LiFT Stem Cell Passaging Reagent can be stored at room temperature for 1 year without loss of activity.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Protocols
Human ips cell passaging protocol using EZ-LiFT™ Reagent, an enzyme-free and chemically defined pluripotent stem cell dissociation reagent.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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