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MAB5284

Sigma-Aldrich

Anti-Chondroitin Sulfate Proteoglycan Antibody, Brain (core protein), clone Cat-301

clone Cat-301, Chemicon®, from mouse

Synonym(s):

CSPG

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

Cat-301, monoclonal

species reactivity

primate, human, rat, gerbil, feline, hamster, guinea pig

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1

NCBI accession no.

NM_001135.2
NM_013227.2

UniProt accession no.

P16112

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... ACAN(176)

General description

The antigen recognized by this clone, CAT-301 is a proteoglycan that is developmentaly regulated, high molecular chondroitin sulphate proteoglycan found on the extracellular surface of mammalian neurons. It is expressed late in development and although no definitive role has been identified for CAT-301, it is believed to have a role in the stabilisation of synaptic structure.

The name derives from the name of the monoclonal antibody originally used to identified by its expression, which was detected immunocytochemicaly



Disruption of the normal patterns of neuronal activity during the critical early postnatal period by physical or biochemical means results in a large and irreversible reduction in levels of CAT-301. Similar intervention in mature animals has no effect



CAT-301 is related to aggrecan from articular cartilage. The CAT-301 antibody immunoreacts with aggrecan, and the chondroitinase treated aggrecan and CAT-301 both have apparent sizes of 550kDa. However CAT- 301 is not glycosylated by keratan sulphate, as aggrecan is.

Specificity

Chondroitin sulfate proteoglycan (CSPG), brain core protein.

Immunogen

Epitope: Brain (core protein)
Feline spinal cord fixed gray matter.

Application

Anti-Chondroitin Sulfate Proteoglycan Antibody, Brain (core protein), clone Cat-301 detects level of Chondroitin Sulfate Proteoglycan & has been published & validated for use in IP, WB, IC, IH.
Immunohistochemistry: 1:500-1:2,000 on 4% paraformaldehyde fixed frozen tissue.

Immunocytochemistry

Immunoblot

Immunoprecipitation

Optimal working dilutions must be determined by the end user.

Physical form

Format: Purified

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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