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MAB3804

Sigma-Aldrich

Anti-53BP1 Antibody, clone BP18

ascites fluid, clone BP18, Chemicon®

Synonym(s):

p53 Binding Protein 1

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

ascites fluid

antibody product type

primary antibodies

clone

BP18, monoclonal

species reactivity

mouse, human

manufacturer/tradename

Chemicon®

technique(s)

immunofluorescence: suitable
western blot: suitable

isotype

IgM

NCBI accession no.

NM_005657.1

UniProt accession no.

Q12888

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... TP53BP1(7158)

Specificity

53BP1

Immunogen

Recombinant human 53BP1 proteins.

Application

Anti-53BP1 Antibody, clone BP18 is a Mouse Monoclonal Antibody for detection of 53BP1 also known as p53 Binding Protein 1 & has been validated in IF & WB.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Western blot. Recognizes a protein of >250 kD. Suggested dilution buffer is TBST. Suggested blocking buffer is 5% non-fat dry milk.

Immunocytochemistry

Immunoprecipitation. Expected size in immunoblot is >250 kD. Suggested lysis buffer is NETN. Suggested capture agent is Protein A + rabbit anti-mouse linker antibody.

Optimal working dilutions must be determined by the end user.

Physical form

Ascites. Liquid.

Storage and Stability

Maintain at -20°C in undiluted aliquots up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Deeksha Munnur et al.
Cell reports, 26(8), 2028-2036 (2019-02-21)
Although poly-ADP-ribosylation (PARylation) of DNA repair factors had been well documented, its role in the repair of DNA double-strand breaks (DSBs) is poorly understood. NR4A nuclear orphan receptors were previously linked to DSB repair; however, their function in the process

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