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Key Documents

HATF04700

Millipore

Immobilon®-NC Transfer Membrane

47 mm diameter disc, 0.45 µm pore size, Triton-free, mixed cellulose esters transfer membrane

Synonym(s):

Western blotting membrane, blotting membrane, nitrocellulose transfer membrane, transfer membrane

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About This Item

UNSPSC Code:
40161507
eCl@ss:
32031602
NACRES:
NB.24

material

mixed cellulose esters (MCE) membrane (surfactant-free)
nitrocellulose membrane
plain filter
white filter

Quality Level

400

feature

hydrophilic

manufacturer/tradename

Immobilon®

parameter

4 L/min-cm2 air flow rate
60 mL/min-cm2 water flow rate
75 °C max. temp.

technique(s)

western blot: suitable

filter diam.

47 mm

thickness

150 μm

refractive index

n/D 1.51

matrix

Immobilon®

pore size

0.45 μm pore size
79 % porosity

capacity

117 μg/cm2 adsorption capacity (insulin)
 160 μg/cm2 adsorption capacity (BSA)
 259 μg/cm2 adsorption capacity (goat IgG)

bubble point

≥2.1 bar, air with water at 23 °C

compatibility

for use with Amido black
for use with CPTS
for use with Colloidal gold
for use with India ink
for use with Ponceau-S red

detection method

chemiluminescent
colorimetric
fluorometric
radioactive

shipped in

ambient

Related Categories

General description

Biologically inert mixtures of cellulose acetate and cellulose nitrate have made MF-Millipore membrane filters one of the most widely used membranes in analytical and research applications.

MF-Millipore filters without Triton surfactant contain minimum amounts of wetting agent and have a lower water extractable content than standard MF-Millipore filters.

Features & Benefits:
- Versatile filter for biological and environmental monitoring applications
- Available in a range of pore sizes, colored black or white, with or without a gridded surface
- Compatible with ethylene oxide, gamma irradiation, and autoclave sterilization methods
Filter Code: HATF
Primary Binding Mechanisms: Electrostatic, hydrophobic

Application

  • Biological solutions
  • Cell contact
  • Very small volumes requiring surfactant-free surfaces
  • Isolation of virus-like particles in wastewater
  • Microplastics analysis grade water
  • Nucleic acid binding, including eDNA

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Flame
GHS02

Signal Word

Danger

Hazard Statements

H228

Hazard Classifications

Flam. Sol. 1

Storage Class Code

4.1B - Flammable solid hazardous materials

WGK

WGK 3

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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