FC010
Human Fibronectin
from human plasma, liquid, 1 mg/mL, purified protein, suitable for cell culture
Synonym(s):
Fibronectin protein
Select a Size
Select a Size
About This Item
Recommended Products
Product Name
Human Plasma Fibronectin Purified Protein, from human plasma, liquid, 1 mg/mL (100 MG pack size is lyophilized), purified protein, suitable for cell culture
biological source
human
Quality Level
Assay
~95% (SDS-PAGE)
form
(liquid: 1MG, 5MG, 10MG pack size
lyophilized: 100 MG pack size)
mol wt
220 kDa
manufacturer/tradename
Chemicon®
concentration
1 mg/mL
technique(s)
cell culture | mammalian: suitable
surface coverage
2—10 μg/cm2
General description
Application
Biochem/physiol Actions
Physical form
Storage and Stability
Analysis Note
Other Notes
1.Determine the amount of HFN needed to coat culture vessels by multiplying the total surface area (cm2) by the desired concentration (μg/mL) of HFN. Recommended amount is 2-10 μg/cm2.
2.Wet the surface of each culture vessel to be coated with a minimum amount of sterile balanced salt solution (serum and protein free) required to cover the entire area.
3.Introduce the proper CO2 atmosphere, if required.
4.Add the calculated amount of HFN to each culture vessel.
5.Allow HFN to adsorb to the surface of the vessel for 5-20 minutes.
6.Remove residual balanced salt solution before proceeding with standard cell culture procedures
Product is filtered using a 0.1 micron filter during production. It is recommended to filter product again before use/after reconstitution.
Legal Information
Disclaimer
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Articles
Attachment Factors for 3-Dimensional Cell Culture
The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues.
Development of a novel serum-free and xeno-free human mesenchymal stem cell (MSC) osteocyte differentiation media.
Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.
Protocols
Dilute fibronectin to the desired concentration. Optimum conditions for attachment are dependent on cell type and application. The typical coating concentration is 1 – 5 ug/cm2.Fibronectin coating protocol, products, and FAQs.
This page covers the indirect co-culture of embryonic stem cells with embryonic fibroblasts.
Information about mesenchyme, specifically mesenchymal stem cell procotols. Step-by-step cell culture protocols for mesenchymal stem cell (MSC) isolation, expansion and differentiation.
Related Content
This page covers the ECM coating protocols developed for four types of ECMs on Millicell®-CM inserts, Collagen Type 1, Fibronectin, Laminin, and Matrigel.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service