70794
BugBuster® GST-Bind Purification Kit
Affinity purification of GST fusion proteins
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About This Item
UNSPSC Code:
41106500
NACRES:
NA.32
manufacturer/tradename
Novagen®
storage condition
OK to freeze
General description
Affinity purification of GST fusion proteins
The BugBuster® GST•Bind<TMSYMBOL></TMSYMBOL> Purification Kit combines the GST•Bind Resin, GST•Bind Buffer Kit reagents and BugBuster Protein Extraction Reagent for convenient preparation of soluble cell extracts and affinity purification of GST•Tag<TMSYMBOL></TMSYMBOL> fusion proteins. BugBuster Protein Extraction Reagent is formulated for the gentle disruption of the cell wall of E. coli, resulting in the liberation of soluble protein. Cells are harvested by centrifugation as usual, followed by suspension in BugBuster Reagent. During a brief incubation, soluble proteins are released. The extract is clarified by centrifugation, which removes cell debris and insoluble proteins. The clarified extract is ready to apply to GST•Bind Resin.
Components
•2 x 100 mlBugBuster Protein Extraction Reagent
•10,000 UBenzonase Nuclease, purity >90%
•10 mlGST•Bind Resin
•pkg/4Chromatography Columns
•2 x 100 ml10X GST Bind/Wash Buffer
•40 ml10X Glutathione Reconstitution Buffer
•1 gGlutathione, Reduced
•10,000 UBenzonase Nuclease, purity >90%
•10 mlGST•Bind Resin
•pkg/4Chromatography Columns
•2 x 100 ml10X GST Bind/Wash Buffer
•40 ml10X Glutathione Reconstitution Buffer
•1 gGlutathione, Reduced
Warning
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
Legal Information
BUGBUSTER is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Flam. Liq. 3
Storage Class Code
3 - Flammable liquids
Flash Point(F)
96.8 °F
Flash Point(C)
36 °C
Certificates of Analysis (COA)
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