480275

β-NGF, Human, Recombinant, NSO Cells

• Synonym(s): β-Nerve Growth Factor, Human, Recombinant
• UNSPSC Code: 12352202
• NACRES: NA.75

Properties

• recombinant: expressed in NSO cells
• Quality Level: 100
• Assay: ≥97% (SDS-PAGE)
• form: lyophilized
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• impurities: ≤100 pg/μg Endotoxin (pg/μg β-NGF)
• shipped in: ambient
• storage temp.: −70°C

Description

General description

Recombinant, human β-nerve growth factor expressed in NSO cells. Involved in neuroimmune interactions and inflammation.

Biochem/physiol Actions

ED₅₀ = 800-1500 pg/ml as measured in a cell proliferation assay using a factor-dependent human erythroleukemic cell line

Warning

Toxicity: Standard Handling (A)

Physical form

Lyophilized from sterile-filtered solution in PBS containing 50 µg BSA/µg β-NGF.

Reconstitution

Following reconstitution, aliquot and freeze (-70°C). Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 3 months at -70°C.

Reconstitute to a concentration ≥1 µg/ml with sterile PBS containing at least 0.1% HSA or BSA.

Other Notes

Leon, A., et al. 1994. Proc. Natl. Acad. Sci. USA91, 3739.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3