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Merck

391340

Millipore

HEPES

≥99% (alkalimetric), solid, pH 6.8-8.2, Molecular Biology

Synonym(s):

HEPES, N-2-Hydroxyethylpiperazine-Nʹ-2-ethanesulfonic Acid, 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)

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About This Item

Empirical Formula (Hill Notation):
C8H18N2O4S
CAS Number:
Molecular Weight:
238.30
Beilstein:
883043
MDL number:
UNSPSC Code:
12161704
NACRES:
NA.25

Pricing and availability is not currently available.

product name

HEPES, Free Acid, Molecular Biology Grade, A zwitterionice N-substituted aminosulfonic acid buffer. Useful in the pH 6.8-8.2 range. Has a pKa of 7.48 at 25°C.

grade

Molecular Biology

Quality Level

Assay

≥99% (alkalimetric)

form

solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

color

white

useful pH range

6.8-8.2

pKa (25 °C)

7.5

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Show Differences

1 of 4

This Item
H8541F3422SRP3044
assay

≥95% (SDS-PAGE)

assay

≥95% (SDS-PAGE)

assay

≥98% (SDS-PAGE and HPLC)

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

recombinant

expressed in HEK 293 cells

recombinant

expressed in HEK 293 cells

recombinant

expressed in E. coli

recombinant

expressed in E. coli

biological source

human

biological source

human

biological source

human

biological source

human

Quality Level

100

Quality Level

300

Quality Level

100

Quality Level

-

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized

General description

A zwitterionice N-substituted aminosulfonic acid buffer. Useful in the pH 6.8-8.2 range. Has a pKa of 7.48 at 25°C.

Application


  • Fluorescent labeling of the root cap cells with the bioactive NBD-S chemical probe based on the cellulose biosynthesis inhibition herbicides: This innovative study demonstrates the application of molecular biology grade HEPES in the formulation of bioactive probes for the fluorescent labeling of root cap cells. Using cellulose biosynthesis inhibition herbicides as a model system, this research provides valuable insights into plant biology, aiding in the understanding of plant cell wall structures and functions. The use of HEPES helps maintain the physiological pH during the experiments, ensuring the stability and effectiveness of the chemical probes used (Tamogami S et al., 2021).

Warning

Toxicity: Standard Handling (A)

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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