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Key Documents

07-892

Sigma-Aldrich

Anti-phospho-Pyk2/CAKβ/FAK2 (Tyr402) Antibody

Upstate®, from rabbit

Synonym(s):

PTK2B, Proline-rich tyrosine kinase 2, Focal adhesion kinase 2, FADK2, Cell adhesion kinase beta, RAFTK, Calcium-dependent tyrosine kinase, CADTK

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human, rat

manufacturer/tradename

Upstate®

technique(s)

immunocytochemistry: suitable
western blot: suitable

isotype

IgG

NCBI accession no.

NM_004103.3
NM_173174.1
NM_173175.1
NM_173176.1

UniProt accession no.

Q14289

shipped in

dry ice

target post-translational modification

phosphorylation (pTyr402)

Gene Information

human ... PTK2B(2185)

Specificity

Pyk2 phosphorylated on tyrosine residue 402

Immunogen

Peptide corresponding to amino acid region encompassing the human and rat phospho-Pyk2/CAKβ/FAK2 (Tyr402).

Application

Immunoblot Analysis: 1:1000 dilutions of this lot detected phospho-Pyk2 (Tyr402) in RIPA lysate of Pyk2 transfected CEF cells
This Anti-phospho-Pyk2/CAKβ/FAK2 (Tyr402) Antibody is validated for use in WB, IC for the detection of phospho-Pyk2/CAKβ/FAK2 (Tyr402).

Quality

Routinely evaluated by immunoblot.

Target description

115 kDa

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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M D Schaller et al.
The Journal of biological chemistry, 272(40), 25319-25325 (1997-10-06)
pp125(FAK) and CAKbeta/Pyk2/CadTK/RAFTK are related protein-tyrosine kinases. It is therefore of interest whether CAKbeta shares some of the properties of pp125(FAK). Using recombinant glutathione S-transferase fusion proteins, we show that the C-terminal domains of both proteins bind paxillin in vitro.

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