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Key Documents

07-843

Sigma-Aldrich

Anti-phospho-IRS1 (Ser616) Antibody

Upstate®, from rabbit

Synonym(s):

IRS-1, Insulin Receptor Substrate 1

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

manufacturer/tradename

Upstate®

technique(s)

immunocytochemistry: suitable
western blot: suitable

isotype

IgG

NCBI accession no.

NM_000208.2
NM_001079817.1

UniProt accession no.

P06213

shipped in

dry ice

target post-translational modification

phosphorylation (pSer616)

Gene Information

human ... INSR(3643)

Specificity

Human IRS1 (Insulin Receptor Substrate 1) phosphorylated on Serine residue 616. Sequence 100% homologous with mouse, rat, and pig.
Predicted to cross-react with mouse and rat based on 100% sequence homology, but have not been tested.

Immunogen

Peptide corresponding to amino acid region encompassing the human, mouse, and rat phospho-IRS-1 (Ser616)

Application

Immunoblot Analysis: 0.5 μg/mL dilutions of this antibody detected phospho-IRS1 (Ser616) in RIPA lysates from TPA treated CHO cells transfected with human IRS1.
Research Category
Signaling
Research Sub Category
Insulin/Energy Signaling
anti-phospho-IRS1 (Ser616) Antibody is an antibody against phospho-IRS1 (Ser616) for use in IC & WB.

Quality

Evaluated by immunoblot.

Target description

165 kDa

Physical form

100 μL of affinity purified rabbit polyclonal IgG in PBS (without Mg2+ and Ca2+), pH 7.3, containing 1.0 mg/mL BSA (IgG, protease free), 0.05% sodium azide and 50% glycerol.
Affinity purified

Storage and Stability

1 year at -20°C.
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/ thaw cycles, which may damage IgG and affect product performance.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Mei-Hsin Hsu et al.
International journal of molecular sciences, 21(10) (2020-05-16)
To examine the effects of maternal resveratrol in rats borne to dams with gestational high-fat diet (HFD)/obesity with or without postnatal high-fat diet. We first tested the effects of maternal resveratrol intake on placenta and male fetus brain in rats
M Zulema Cabail et al.
Nature communications, 6, 6406-6406 (2015-03-12)
The insulin receptor (IR) and insulin-like growth factor-1 receptor (IGF1R) are highly related receptor tyrosine kinases with a disulfide-linked homodimeric architecture. Ligand binding to the receptor ectodomain triggers tyrosine autophosphorylation of the cytoplasmic domains, which stimulates catalytic activity and creates

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