05-747
Anti-phospho-MEK1 (Ser218/222)/MEK2 (Ser222/226) Antibody, rabbit monoclonal
Upstate®, from rabbit
Synonym(s):
Anti-CFC3, Anti-MAPKK1, Anti-MEK1, Anti-MEL, Anti-MKK1, Anti-PRKMK1
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
rabbit
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
monoclonal
species reactivity
human, mouse, rat
manufacturer/tradename
Upstate®
technique(s)
western blot: suitable
isotype
IgG
UniProt accession no.
shipped in
dry ice
target post-translational modification
phosphorylation (pSer218/pSer222)
Gene Information
human ... MAP2K1(5604)
Specificity
phosphorylated MEK1/MEK2
Immunogen
Peptide containing DpSMANpSF in which pS corresponds to phosphorylated amino acids 218 and 222 of human MEK1
Application
Detect phospho-MEK1 (Ser218/222)/MEK2 (Ser222/226) using this Anti-phospho-MEK1 (Ser218/222)/MEK2 (Ser222/226) Antibody validated for use in WB.
Research Category
Signaling
Signaling
Research Sub Category
MAP Kinases
MAP Kinases
Quality
routinely evaluated by immunoblot on RIPA lysates from PC-12 cells that have been treated with B-Raf
Target description
45kDa
Linkage
Replaces: 04-1017
Physical form
0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
Format: Purified
Protein A purified
Storage and Stability
2 years at -20°C
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Negative regulation of MAPKK by phosphorylation of a conserved serine residue equivalent to Ser212 of MEK1.
Gopalbhai, Kailesh, et al.
The Journal of Biological Chemistry, 278, 8118-8125 (2003)
Gonzalo L Gonzalez-Del Pino et al.
Proceedings of the National Academy of Sciences of the United States of America, 118(36) (2021-09-03)
The RAF/MEK/ERK pathway is central to the control of cell physiology, and its dysregulation is associated with many cancers. Accordingly, the proteins constituting this pathway, including MEK1/2 (MEK), have been subject to intense drug discovery and development efforts. Allosteric MEK
C Hodge et al.
The Journal of biological chemistry, 273(47), 31327-31336 (1998-11-13)
Growth hormone (GH), a major regulator of normal body growth and metabolism, regulates cellular gene expression. The transcription factors Elk-1 and Serum Response Factor are necessary for GH-stimulated transcription of c-fos through the Serum Response Element (SRE). GH stimulates the
J C S Ho et al.
Oncogene, 35(7), 897-907 (2015-06-02)
HAMLET (Human Alpha-lactalbumin Made LEthal to Tumor cells) kills tumor cells broadly suggesting that conserved survival pathways are perturbed. We now identify nucleotide-binding proteins as HAMLET binding partners, accounting for about 35% of all HAMLET targets in a protein microarray
The 5-HT 2A serotonin receptor enhances cell viability, affects cell cycle progression and activates MEK-ERK1/2 and JAK2-STAT3 signalling pathways in human choriocarcinoma cell lines.
Oufkir T, Arseneault M, Sanderson JT, Vaillancourt C
Placenta null
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