Custom DNA Oligos Modifications

We offer over 200 modifications for both DNA and RNA oligonucleotides and probes. We can attach fluorescent dyes to oligos at the 5'-end, 3'-end and internal positions. Our product offering includes common dye modifiers such as 6-FAM™, HEX™, and TET™, as well as exotic modifications.

In addition to standard fluorescent modifications, we have the capabilities to provide dual-labeled probes including Molecular Beacons, LightCycler^® Probes, and Scorpions^® Probes.

• Minimum Yield Guarantees
• Shipping Schedule
• Quality Control

Modification Categories

• Fluorescent
• Attachment
• Binding
• Spacer
• Analog
• Intercalation
• Antisense
• Phosphorothioates

Fluorescent	Positions	Excitation Maximum (nm)	Emission Maximum (nm)	Fluorescent Color
6-FAM™ (fluorescein)	5' End, 3' End	495	520	Green
ROX™	5' End, 3' End	586	610	Orange
Cyanine 3	5' End, 3' End	549	566	Green-Yellow
Cyanine 5	5' End, 3' End	646	669	Red
Cyanine 5.5	5' End	675	694	Red
6-FAM dT	5' End, Internal	495	520	Green
HEX™	5' End	535	556	Green
JOE™	5' End, 3' End	529	555	Green
6-Carboxy-rhodamine 6G™	5' End, 3' End	524	550	Green
TAMRA	3' End	557	583	Green-Yellow
TAMRA NHS Ester	5' End	557	583	Yellow
TET™	5' End	521	536	Green
TxRd (Sulforhodamine 101-X)	5' End, 3' End	597	616	Red-Orange
A488 (Sulfonated Fluorescein 488)	5' End, 3' End	495	519	Green
WellRED D2-PA	5' End	750	770	Red-Infrared
WellRED D3-PA	5' End	685	706	Red
WellRED D4-PA	5' End	650	670	Red
Attachment	Positions	Purifications
Amine
DMS(O)MT-Amino-Modifier-C6	5' End	Cartridge, HPLC
Amino-Modifier-C3-TFA	5' End	Desalt, HPLC
Amino-Modifier-C12	5' End	Cartridge, HPLC
Amino-Modifier-C6-TFA	5' End	Desalt, PAGE
Amino-dT	5' End	Cartridge, HPLC
Amino-Modifier-5	5' End	Cartridge, HPLC
Amino-Modifier-C2-dT	Internal	Desalt, Cartridge, HPLC, PAGE
Amino-Modifier-C6-dT	Internal	Desalt, Cartridge, HPLC, PAGE
3'-Amino-Modifier-C7	3' End	Desalt, Cartridge, HPLC, PAGE
Glycerol
Glyceryl	3' End	Desalt, Cartridge, HPLC, PAGE
Phosphate
Phosphate	3', 5' End	Desalt, PAGE (5') Desalt, Cartridge, HPLC, PAGE (3')
Phosphate II	5' End	Cartridge, HPLC
Thiol
5'-Thiol-Modifier C6 S-S	5' End, Internal	Cartridge, HPLC
3'-Thiol-Modifier-C3 S-S	3' End	Desalt, Cartridge, HPLC, PAGE
Binding	Positions	Purifications
Biotin
5'-Biotin	5' End	Desalt, Cartridge, HPLC, PAGE
Biotin	Internal	HPLC, PAGE
Biotin-dT	5' End, Internal	HPLC, PAGE
Biotin-TEG	5' End, Internal	Cartridge, HPLC
3'-Biotin-TEG	3' End	HPLC, PAGE
Digoxigenin
Digoxigenin	5' End, 3' End	HPLC
Dinitrophenol
2,4-Dinitrophenyl-TEG	5' End, Internal	HPLC
Spacer	Positions	Purifications
Spacer
Spacer 9	5' End, Internal	Desalt, Cartridge, HPLC, PAGE
Spacer 12	5' End, Internal	Desalt, Cartridge, HPLC, PAGE
Spacer 18	5' End, Internal	Desalt, Cartridge, HPLC, PAGE
Spacer C3	5' End, Internal	Desalt, Cartridge, HPLC, PAGE
3'-Spacer-C3	3' End	Desalt, Cartridge, HPLC, PAGE
Analog	Positions	Purifications
Chain Terminating
3'-dA	3' End	Desalt, Cartridge, HPLC, PAGE
3'-dG	3' End	Desalt, Cartridge, HPLC, PAGE
3'-dC	3' End	Desalt, Cartridge, HPLC, PAGE
3'-dT	3' End	Desalt, Cartridge, HPLC, PAGE
Chemotherapeutic
Cytosine Arabinoside (araC)	Internal	Desalt, Cartridge, HPLC, PAGE
2',3'-dideoxycytidine (ddC)	3' End	Desalt, Cartridge, HPLC, PAGE
Contamination Prevention
Ribo U	3' End	Desalt, Cartridge, HPLC, PAGE
Crosslinking
8-Br-dA	5' End, Internal	Desalt, Cartridge, HPLC, PAGE
8-Br-dG	5' End, Internal	Desalt, Cartridge, HPLC, PAGE
5-Br-dC	5' End, Internal	Desalt, Cartridge, HPLC, PAGE
5-Br-dU	5' End, Internal	Desalt, Cartridge, HPLC, PAGE
5-F-dU	5' End, Internal	Desalt, Cartridge, HPLC, PAGE
5-I-dC	5' End, Internal	Desalt, Cartridge, HPLC, PAGE
5-I-dU	5' End, Internal	Desalt, Cartridge, HPLC, PAGE
2-Thio-dT	5' End, Internal	Desalt, Cartridge, HPLC, PAGE
4-Thio-dT	5' End, Internal	Desalt, Cartridge, HPLC, PAGE
6-Thio-dG	5' End, Internal	Desalt, Cartridge, HPLC, PAGE
Damage and Repair
2'-Deoxypseudouridine	5' End, Internal	Desalt, Cartridge, HPLC, PAGE
5,6-Dihydro-dT	5' End, Internal	Desalt, Cartridge, HPLC, PAGE
5,6-Dihydro-dU	5' End, Internal	Desalt, Cartridge, HPLC, PAGE
5-OH-dC	5' End, Internal	Desalt, Cartridge, HPLC, PAGE
5-OH-dU	5' End, Internal	Desalt, Cartridge, HPLC, PAGE
8-Oxo-dA	5' End, Internal	Desalt, Cartridge, HPLC, PAGE
8-Oxo-dG	5' End, Internal	Desalt, Cartridge, HPLC, PAGE
Thymidine Glycol	5' End, Internal	PAGE
TMP-F-dU, F-dC	Internal	Desalt, Cartridge, HPLC, PAGE
dUracil	5' End, Internal, 3' End	Desalt, Cartridge, HPLC, PAGE
Degenerate
2'-Deoxynebularine	5' End, Internal	Cartridge, HPLC, PAGE
Derivative K (dK)	5' End, Internal, 3' End	Desalt, Cartridge, HPLC, PAGE
Derivative P (dP)	5' End, Internal, 3' End	Desalt, Cartridge, HPLC, PAGE
Inosine	5' End, Internal, 3' End	Desalt, Cartridge, HPLC, PAGE
5-Nitroindole	5' End, Internal, 5' End	Desalt, Cartridge, HPLC, PAGE
3-Nitropyrrole	5' End, Internal, 3' End	Desalt (Internal only), Cartridge, HPLC, PAGE
Duplex Stabilizing
2,6-Diaminopurine	5' End, Internal	Desalt, Cartridge, HPLC, PAGE
5-Me-dC	5' End, Internal	Desalt, Cartridge, HPLC, PAGE
Fluorescent
2-Aminopurine	5' End, Internal	Desalt, Cartridge, HPLC, PAGE
Etheno-dA	5' End, Internal	Desalt, Cartridge, HPLC, PAGE
Hybridization Specificity
*Locked Nucleic Acid	5' End, Internal, 3' End	Cartridge, HPLC, PAGE
Methylation Mutagenesis
N6-Me-dA	5' End, Internal	Desalt, Cartridge, HPLC, PAGE
O6-Me-dG	5' End, Internal	Desalt, Cartridge, HPLC, PAGE
O4-Me-dT	5' End, Internal	Desalt, Cartridge, HPLC, PAGE
No Base
dSpacer	Internal	Desalt, Cartridge, HPLC, PAGE
siRNA Guide Strand Selection
5'-OMe-dT	5' End	Desalt, HPLC, PAGE
Structure and Function
7-Deaza-dA	5' End, Internal	Desalt, Cartridge, HPLC, PAGE
7-Deaza-dG	5' End, Internal	Desalt, Cartridge, HPLC, PAGE
7-Deaza-dX	5' End, Internal	Desalt, Cartridge, HPLC, PAGE
7-Deaza-8-Aza-dA	5' End, Internal	Desalt, Cartridge, HPLC, PAGE
dA-5' (Inverse Oligo)	5' End, Internal, 3' End	Desalt, Cartridge, HPLC, PAGE
dG-5' (Inverse Oligo)	5' End, Internal, 3' End	Desalt, Cartridge, HPLC, PAGE
dC-5' (Inverse Oligo)	5' End, Internal, 3' End	Desalt, Cartridge, HPLC, PAGE
dT-5' (Inverse Oligo)	5' End, Internal, 3' End	Desalt, Cartridge, HPLC, PAGE
Translation Termination
Puromycin	3' End	Desalt, Cartridge, HPLC, PAGE
Intercalation	Positions	Purifications
Acridine
Acridine	Internal	Desalt, Cartridge, HPLC, PAGE
Psoralen
C2	5' End	Desalt, Cartridge, HPLC, PAGE
C6	5' End	Desalt, Cartridge, HPLC, PAGE
Antisense	Positions	Purifications
Cholesterol
Cholesteryl-TEG	5' End, Internal	Cartridge, HPLC
3'-Cholesteryl-TEG	3' End	Desalt, Cartridge, HPLC, PAGE
Methyl RNA
2'-OMe-RNA A	5' End, Internal, 3' End	Desalt, Cartridge, HPLC, PAGE
2'-OMe-RNA G	5' End, Internal, 3' End	Desalt, Cartridge, HPLC, PAGE
2'-OMe-RNA C	5' End, Internal, 3' End	Desalt, Cartridge, HPLC, PAGE
2'-OMe-RNA U	5' End, Internal, 3' End	Desalt, Cartridge, HPLC, PAGE
Thiophosphate
Thiophosphate (S-Oligo)	5' End, Internal, 3' End	Desalt, Cartridge (5' End, Internal) Desalt, Cartridge, HPLC, PAGE (3' End)
2' → 5' Synthesis
dA-5'	5' End, Internal, 3' End	Desalt, Cartridge, HPLC, PAGE
dG-5'	5' End, Internal, 3' End	Desalt, Cartridge, HPLC, PAGE
dC-5'	5' End, Internal, 3' End	Desalt, Cartridge, HPLC, PAGE
dT-5'	5' End, Internal, 3' End	Desalt, Cartridge, HPLC, PAGE

*Locked Nucleic Acid in oligonucleotides other than qPCR probes is available in all countries except for the United States.

Phosphorothioates (or S-oligos)

Phosphorothioates (or S-oligos) are a variant of normal DNA in which one of the non-bridging oxygens is replaced by sulfur. The sulfurization of the internucleotide bond creates a molecule that is resistant to cellular nuclease degradation. Sulfur bonds can be introduced at the 5’, 3’, or internal positions.

Minimum Yield Guarantees

Modified Oligos (O.D./µg)*
Scale (µmole)	Desalt	Cartridge	HPLC	PAGE
0.05	2/60	0.4/12	0.4/12	0.2/6
0.2	5/150	1/30	1/30	0.4/12
1.0	16/480	5/150	5/150	2/60
10	160/4,800	NA	52/1,560	NA
15	240/7,200	NA	76/2,280	NA

*Guarantees are for 20 mers or longer. Oligos shorter than 20 bases will have fewer ODs. Microgram amounts are calculated based on a 30 μg/OD value. If the μg/OD value is less than 30, the reported μg value will be proportionally less. Phosphorothioated yields are 70% of the above stated values. Post-synthesis modifications may yield 50% less than the above stated values.

Non-Modified Phosphorothioates (S-Oligos) (O.D./µg )*
Scale (µmole)	Desalt	Cartridge	HPLC	PAGE
0.05	3.5/105	0.7/21	0.7/21	0.35/10.5
0.2	8.4/252	2.1/63	1.75/52.5	0.7/21
1.0	28/840	8.4/252	9.1/273	3.5/105
10	280/8,400	NA	91/2,730	NA
15	420/12,600	NA	133/3,990	NA

*Guarantees are for 20 mers or longer. Oligos shorter than 20 bases will have fewer ODs. Microgram amounts are calculated based on a 30 μg/OD value. If the μg/OD value is less than 30, the reported μg value will be proportionally less.

Shipping Schedule

Purification	Desalt	Cartridge	HPLC	PAGE
Standard Modifications	5-7 days or less	5-7 days or less	5-7 days or less	Inquire
Post-Synthesis Modifications	7-10 days	7-10 days	7-10 days	Inquire

*For complete label license information, please visit sigmaaldrich.com/oligolicenses.