- ORAI channels are critical for receptor-mediated endocytosis of albumin.
ORAI channels are critical for receptor-mediated endocytosis of albumin.
Nature communications (2017-12-06)
Bo Zeng, Gui-Lan Chen, Eliana Garcia-Vaz, Sunil Bhandari, Nikoleta Daskoulidou, Lisa M Berglund, Hongni Jiang, Thomas Hallett, Lu-Ping Zhou, Li Huang, Zi-Hao Xu, Viji Nair, Robert G Nelson, Wenjun Ju, Matthias Kretzler, Stephen L Atkin, Maria F Gomez, Shang-Zhong Xu
PMID29203863
ZUSAMMENFASSUNG
Impaired albumin reabsorption by proximal tubular epithelial cells (PTECs) has been highlighted in diabetic nephropathy (DN), but little is known about the underlying molecular mechanisms. Here we find that ORAI1-3, are preferentially expressed in PTECs and downregulated in patients with DN. Hyperglycemia or blockade of insulin signaling reduces the expression of ORAI1-3. Inhibition of ORAI channels by BTP2 and diethylstilbestrol or silencing of ORAI expression impairs albumin uptake. Transgenic mice expressing a dominant-negative Orai1 mutant (E108Q) increases albuminuria, and in vivo injection of BTP2 exacerbates albuminuria in streptozotocin-induced and Akita diabetic mice. The albumin endocytosis is Ca
MATERIALIEN
Produktnummer
Marke
Produktbeschreibung
Sigma-Aldrich
Anti-Kaninchen-IgG-(Gesamtmolekül)-Peroxidase in Ziege hergestellte Antikörper, affinity isolated antibody
Sigma-Aldrich
Anti-Kaninchen-IgG-Antikörper der Ziege, Biotin-SP-Konjugat, Chemicon®, from goat