Generation of a TLE3 heterozygous knockout human embryonic stem cell line using CRISPR-Cas9.

Generation of a TLE3 heterozygous knockout human embryonic stem cell line using CRISPR-Cas9.

Stem cell research (2016-11-24)

Anne M Bara, Angelica Messana, Amanda Herring, Dane Z Hazelbaker, Kevin Eggan, Lindy E Barrett

ZUSAMMENFASSUNG

Here, we generated a monoallelic mutation in the TLE3 (Transducin Like Enhancer of Split 3) gene using CRISPR-Cas9 editing in the human embryonic stem cell (hESC) line WA01. The heterozygous knockout cell line, TLE3-447-D08-A01, displays partial loss of TLE3 protein expression while maintaining pluripotency, differentiation potential and genomic integrity.

MATERIALIEN

Produktnummer

Marke

Produktbeschreibung

11836153001

Roche

cOmplete^™ Mini Proteasehemmer-Cocktail, Tablets provided in a glass vial

A2547

Sigma-Aldrich

Monoklonaler Anti-Aktin-Antikörper , α-Glattmuskel, clone 1A4, ascites fluid

P7255

Sigma-Aldrich

Puromycin -dihydrochlorid aus Streptomyces alboniger, ≥98% (HPLC), powder

A8452

Sigma-Aldrich

Monoclonal Anti-α-Fetoprotein (AFP) antibody produced in mouse, ascites fluid, clone C3