- Oncogenic PIK3CA induces centrosome amplification and tolerance to genome doubling.
Oncogenic PIK3CA induces centrosome amplification and tolerance to genome doubling.
Nature communications (2017-11-25)
Inma M Berenjeno, Roberto Piñeiro, Sandra D Castillo, Wayne Pearce, Nicholas McGranahan, Sally M Dewhurst, Valerie Meniel, Nicolai J Birkbak, Evelyn Lau, Laurent Sansregret, Daniele Morelli, Nnennaya Kanu, Shankar Srinivas, Mariona Graupera, Victoria E R Parker, Karen G Montgomery, Larissa S Moniz, Cheryl L Scudamore, Wayne A Phillips, Robert K Semple, Alan Clarke, Charles Swanton, Bart Vanhaesebroeck
PMID29170395
ZUSAMMENFASSUNG
Mutations in PIK3CA are very frequent in cancer and lead to sustained PI3K pathway activation. The impact of acute expression of mutant PIK3CA during early stages of malignancy is unknown. Using a mouse model to activate the Pik3ca
MATERIALIEN
Produktnummer
Marke
Produktbeschreibung
Sigma-Aldrich
Anti-phospho-Histon H2A.X (Ser139)-Antikörper, Klon JBW301, clone JBW301, Upstate®, from mouse
Sigma-Aldrich
Anti-β-Actin-Antikörper, Maus monoklonal, clone AC-15, purified from hybridoma cell culture
Sigma-Aldrich
Anti-α-Tubulin-Antikörper, monoklonaler Antikörper der Maus, clone B-5-1-2, purified from hybridoma cell culture
Sigma-Aldrich
Trypsin -Lösung aus Schweinepankreas, 1 ×, sterile-filtered, BioReagent, suitable for cell culture, 2.5 g porcine trypsin per liter in Hanks′ Balanced Salt Solution with phenol red
Sigma-Aldrich
Monoclonal Anti-α-Actinin antibody produced in mouse, clone BM-75.2, ascites fluid