Direkt zum Inhalt
Merck
  • Simultaneous determination of vitamin K1, vitamin K1 2,3-epoxide and menaquinone-4 in human plasma by high-performance liquid chromatography with fluorimetric detection.

Simultaneous determination of vitamin K1, vitamin K1 2,3-epoxide and menaquinone-4 in human plasma by high-performance liquid chromatography with fluorimetric detection.

Journal of chromatography (1988-08-19)
K Hirauchi, T Sakano, T Nagaoka, A Morimoto
ZUSAMMENFASSUNG

A highly sensitive method for measuring endogenous vitamin K1, menaquinone-4 (which is one of the K2 vitamins) and vitamin K1 2,3-epoxide in human plasma was developed, based on high-performance liquid chromatography with coulometric reduction and fluorimetric detection, following extraction from plasma and purification on a Sep-Pak silica cartridge. The detection limits of vitamin K1, menaquinone-4 and vitamin K1 2,3-epoxide were 5, 5 and 8 pg per injection for the standard substances and 30, 30 and 50 pg/ml in human plasma, respectively.

MATERIALIEN
Produktnummer
Marke
Produktbeschreibung

Sigma-Aldrich
Vitamin K1 2,3-epoxid, ≥98.0% (HPLC)