- Enhanced accumulation of secondary metabolites in hairy root cultures of Scutellaria lateriflora following elicitation.
Enhanced accumulation of secondary metabolites in hairy root cultures of Scutellaria lateriflora following elicitation.
Biotechnology letters (2012-05-29)
Agata Wilczańska-Barska, Aleksandra Królicka, Daniel Głód, Magdalena Majdan, Anna Kawiak, Mirosława Krauze-Baranowska
PMID22639092
ZUSAMMENFASSUNG
Hairy root cultures of Scutellaria lateriflora were established using Agrobacterium rhizogenes A4 and produced acteoside (18.5 mg g(-1) dry wt), baicalin (14.5 mg g(-1) dry wt) and wogonoside (12 mg g(-1) dry wt). Yeast extract (50 μg ml(-1)) increased acteoside production 1.4-fold and flavone production 1.7-fold after 7 and 14 days of elicitation. Addition of Pectobacterium carotovorum lysate in the stationary phase of the hairy root culture stimulated only the accumulation of wogonin to 30 mg g(-1) dry wt. The production of wogonin in hairy roots could be associated with its role as a phytolaexin.