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Merck

H0413

Millipore

HIS-Select® Filter Plate

Synonym(e):

His-tag filtered microplate

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About This Item

UNSPSC-Code:
41121800
NACRES:
NB.24

Qualität

for molecular biology

Form

plate

Lagertemp.

2-8°C

Allgemeine Beschreibung

The HIS-Select® Filter Plate is an immobilized metal-ion affinity chromatography (IMAC) product that allows rapid purification of small-scale crude cell extracts containing histidine-tagged proteins. The HIS-Select® Filter Plate contains 20 μm, spherical silica particles (100 nm pore size) with a hydrophilic layer.

The HIS-Select® Filter Plate is selective for recombinant proteins with histidine tags and exhibits very low non-specific binding of other proteins. The selectivity can be modulated with the inclusion of imidazole during chromatography. The binding capacity of the HIS-Select® Filter Plate is typically > 2 mg per well as determined with an approximately 30 kDa histidine-tagged protein.

Anwendung

The HIS-Select® Filter Plate is suitable for purification of recombinant proteins with histidine tags.

Leistungsmerkmale und Vorteile

  • High Selectivity for his-tagged proteins
  • Ready-to-use plate format
  • Compatible with CelLytic cell lysis reagents

Rechtliche Hinweise

Covered by US Patent No 6,623,655 and are sold for research use only. Commercial use requires additional licenses.
CelLytic is a trademark of Sigma-Aldrich Co. LLC
HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany

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Piktogramme

Health hazard

Signalwort

Danger

Gefahreneinstufungen

Aquatic Chronic 3 - Carc. 1A Inhalation - Muta. 2 - Repr. 1B - Resp. Sens. 1 - Skin Sens. 1 - STOT RE 1 - STOT RE 2 Inhalation

Zielorgane

Respiratory Tract

Lagerklassenschlüssel

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


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