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Merck

GE28-9909-46

Sigma-Aldrich

Superdex® 200 Steigerung 3,2/300

column L × I.D. 30 cm × 3.2 mm, 8.6 μm particle size

Synonym(e):

Superdex 200 Increase

Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise


About This Item

UNSPSC-Code:
41115700
NACRES:
SB.52

Materialien

glass column
titanium frit

Produktlinie

Superdex®

Mol-Gew.

10000-600000 Da (globular proteins)

Verpackung

1 ea of

Parameter

<0.15 ml/min flow rate
3.0 Mpa (652 psi)
4-50 mL sample volume

Bettvolumen

~2.4 mL

Säule L × ID

30 cm × 3.2 mm

Matrix

agarose-dextran composite
polymeric

Partikelgröße

8.6 μm

cleaning

1-14

working range

3-12

Trenntechnik

size exclusion (SEC)

Allgemeine Beschreibung

Superdex™ 200 Increase size exclusion chromatography columns are well suited for high-resolution analysis and small-scale purification of monoclonal antibodies (mAb) and other biomolecules with Mr~ 10 000 to ~ 600 000.

Superdex™ 200 Increase is a next-generation, dextran-agarose composite matrix for SEC. With smaller and more rigid beads than their predecessors, Superdex™ Increase columns deliver higher resolution purification in shorter run times.

Superdex™ 200 Increase has a selectivity curve optimized for excellent resolution of antibodies and macromolecules within the range Mr ~ 100 000 to ~ 300 000. It is well suited for detection and separation of antibody monomers, dimers, and aggregates present in monoclonal antibody preparations. The outstanding resolving power of this resin facilitates protein characterization, including analysis of antibody size variants, assessment of membrane protein size homogeneity, and the study of protein-protein interactions.

Superdex™ 200 Increase is optimized to give high resolution separation for proteins with molecular weights in the range Mr ~ 100 000 to ~ 300 000. Superose™ 6 Increase provides a complementary fractionation range, resolving very large protein complexes and macromolecules that Superdex 200 Increase cannot separate.

This agarose-based resin is alkali-resistant and supports cleaning-in-place (CIP) procedures. This capability allows the same column to be used for different proteins, with minimal risk of carry-over between samples.

Leistungsmerkmale und Vorteile

  • Enhanced performance: improved resolution and runtime compared to predecessor Superdex™ 200.
  • Very high resolution: small bead size and narrow particle size distribution provide high resolution, for high protein purity.
  • High flow rates: rigid beads give excellent pressure/flow properties.

Eignung

Langfristige Verwendung: alle gebräuchlichen wässrigen Puffer, bis zu 8 M Harnstoff, ionische und nicht-ionische Detergenzien, bis zu 6 M Guanidinhydrochlorid
für Vorbereitungs- und Analysezwecke

Lagerung und Haltbarkeit

Bei 4 bis 30 °C aufbewahren (20 % Ethanol)
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Empfohlene Produkte

LiChropur-Reagenzien eignen sich ideal für die HPLC- oder LC-MS-Analyse

Rechtliche Hinweise

Superdex is a registered trademark of Cytiva

Piktogramme

Flame

Signalwort

Warning

H-Sätze

Lagerklassenschlüssel

3 - Flammable liquids


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Artikel

Pressure is generated by the flow through the chromatographic system. For optimal chromatography functionality, it is important to understand the principle of the pressure drop over the different parts of a system.

This page shows how to perform a purification of His-tagged membrane proteins.

Protokolle

Superdex from GE Healthcare are SEC media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a superdex column.

Samples for chromatographic purification should be clear and free from particulate matter. Simple steps to clarify a sample before beginning purification will avoid clogging the column, can reduce the need for stringent washing procedures, and can extend the life of the chromatographic medium.

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