E4643

Heparin-Binding EGF-Like Growth Factor human

≥97% (SDS-PAGE and N-terminal analysis), recombinant, expressed in baculovirus infected Sf21 cells, lyophilized powder, suitable for cell culture

• Synonym(e): hHB-EGF, HB-EGF, Heparin-Binding Epidermal Growth Factor-Like Growth Factor
• MDL-Nummer: MFCD00212935
• UNSPSC-Code: 12352202
• NACRES: NA.77

Eigenschaften

• Produktbezeichnung: Heparin-Binding EGF-Like Growth Factor human, HB-EGF, recombinant, expressed in baculovirus infected Sf21 cells, lyophilized powder, suitable for cell culture
• Biologische Quelle: human
• Qualitätsniveau: 100
• Rekombinant: expressed in baculovirus infected Sf21 cells
• Assay: ≥97% (SDS-PAGE and N-terminal analysis)
• Form: lyophilized powder
• Wirksamkeit: ≤3 ng/mL
• Qualität: endotoxin tested
• Mol-Gew.: 9.5 kDa
• Verpackung: pkg of 50 and 250 μg
• Lagerbedingungen: avoid repeated freeze/thaw cycles
• Methode(n): cell culture | mammalian: suitable
• UniProt-Hinterlegungsnummer: Q99075
• Lagertemp.: −20°C
• Angaben zum Gen: human ... HBEGF(1839) , HBEGF(1839)

Beschreibung

Biochem./physiol. Wirkung

Heparin-binding epidermal growth factor (HB-EGF), like BTC, is a heparin-binding EGF family member. Natural soluble HB-EGF is a 19 kDa glycoprotein of multiple forms due to heterogeneous truncations. Soluble HB-EGF is mitogenic and chemotactic for fibroblasts, keratinocytes, and smooth muscle cells but not capillary endothelial cells. Production of HB-EGF is detected in monocytes, macrophages, and vascular and aortic smooth muscle cells (SMC). HB-EGF may play a role in the pathogenesis of atherosclerosis. It may also be an effector of Jun-induced oncogenic transformation. HB-EGF binds to EGFR with high affinity and to ErbB with moderate affinity.

Physikalische Form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 2.5 mg bovine serum albumin.

Hinweis zur Analyse

The biological activity is measured by its ability to stimulate ³H-thymidine incorporation in the EGF-responsive BALB/3T3 cells.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)