HCT-8

90032006, human intestine, Epithelial

• UNSPSC-Code: 41106514

Eigenschaften

• Produktbezeichnung: HCT-8, 90032006
• Biologische Quelle: human intestine
• Wachstumsmodus: Adherent
• Karyotyp: 2n = 46, pseudodiploid
• Morphologie: Epithelial
• Produkte: Carcinoembryonic antigen (CEA)
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human ileocecal adenocarcinoma

Beschreibung der Zelllinie

From a 67 year old male. The four cell lines: DLD-1 (90102540), HCT-15 (91030712), HCT-8 (90032006) and HRT-18 (86040306) have been shown to have a common genetic origin.

Anwendung

Tumourigenicity studies

Nährmedium

RPMI 1640 + 2mM Glutamine + 1mM Sodium Pyruvate (NaP) + 10% Horse Serum (HS) or 5% Horse Serum (HS) + 5% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 1-2x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Sonstige Hinweise

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