MAB3786-C

Anti-APC Antibody, CT, clone C-APC Antibody 28.9, Ascites Free

clone C-APC 28.9, from mouse

• Synonym(e): Adenomatous polyposis coli protein, Deleted in polyposis 2.5, Protein APC
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 100
• Antikörperform: purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: C-APC 28.9, monoclonal
• Speziesreaktivität: human
• Methode(n): immunocytochemistry: suitable; immunofluorescence: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• Isotyp: IgG1κ
• NCBI-Hinterlegungsnummer: NP_000029
• UniProt-Hinterlegungsnummer: P25054
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... APC(324)

Beschreibung

Allgemeine Beschreibung

Adenomatous polyposis coli protein (UniProt P25054; also known as Deleted in polyposis 2.5, Protein APC) is encoded by the APC (also known as DP2.5) gene (Gene ID 324) in human. The multifunctional tumor suppressor APC is a large scaffold protein that contains multiple armadillo repeat (ARM) domains (a.a. 453-767 of human APC spliced isoform 1) for mediating various cellular functions, including Wnt signal regulation, cell adhesion, and chromosomal segregation during mitosis. APC, AXIN, casein kinase 1 (CK1) and glycogen synthase kinase 3 (GSK3) constitute the core proteins of the β-catenin destruction complex that functions as the negative regulator against Wnt signaling transduction. Mice with Apc gene mutations develop adenomas in the intestine due to Wnt hyperactivation, and functional loss of APC due to gene mutations is the hallmark of human colorectal cancers (CRC). APC also directs cholinergic synapse assembly between neurons and is commonly employed as a marker for monitoring stem cells neuronal differentiation.

Spezifität

Recognizes human APC, molecular weight is approximately 310 kDa.

Immunogen

Epitope: C-terminal region.

Human APC C-terminal fusion with MBP (maltose binding protein).

Anwendung

Research Category
Epigenetik & nukleäre Funktionen

Research Sub Category
Zellzyklus, DNA-Replikation & -Reparatur

Anti-APC Antibody, CT, clone C-APC Antibody 28.9, Ascites Free is an antibody against APC for use in Immunohistochemistry (Paraffin), Immunocytochemistry, Immunofluorescence, Western Blotting.

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected APC in human kidney and cardiac muscle.
Immunocytochemistry Analysis: A representative detected an increased APC expression in human eyelid adipose-derived stem cells (hEASCs) after neuroinduction at passage 8 (Zhou, J., et al. (2014). J. Cell. Mol. Med. 18(2):326-343).
Immunofluorescence Analysis: A representative lot detected a granular APC staining pattern at a subapical region of epithelial cells in fallopian mucosa by fluorescent immunohistochemistry staining of paraffin-embedded, paraformaldehyd-fixed fallopian tube tissue sections (Kessler, M., et al. (2012). Am. J. Pathol. 180(1):186-198).

Qualität

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected APC in human cerebral cortex tissue.

Zielbeschreibung

311.6 kDa calculated.

Physikalische Form

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Sonstige Hinweise

Concentration: Please refer to lot specific datasheet.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable