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Key Documents

04-281

Sigma-Aldrich

Anti-phospho-EGFR (Thr669) Antibody, clone 5F10

clone 5F10, Upstate®, from mouse

Synonym(e):

ERBB, ERBB1, epidermal growth factor receptor

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About This Item

UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Biologische Quelle

mouse

Qualitätsniveau

100

Antikörperform

purified immunoglobulin

Antikörper-Produkttyp

primary antibodies

Klon

5F10, monoclonal

Speziesreaktivität

human

Hersteller/Markenname

Upstate®

Methode(n)

western blot: suitable

Isotyp

IgG1

NCBI-Hinterlegungsnummer

NM_005228.3
NM_201282.1
NM_201283.1
NM_201284.1

UniProt-Hinterlegungsnummer

P00533

Versandbedingung

wet ice

Posttranslationale Modifikation Target

phosphorylation (pThr669)

Angaben zum Gen

human ... EGFR(1956)

Spezifität

Recognizes EGFR when phosphorylated at Threonine 669.

Immunogen

KLH-conjugated synthetic peptide encompassing the surrounding amino acids of Thr 669 in human EGFR.

Anwendung

Research Category
Zelluläre Signaltransduktion
Research Sub Category
Wachstumsfaktoren & -rezeptoren
Anti-phospho-EGFR (Thr669) Antibody, clone 5F10 detects level of phospho-EGFR (Thr669) & has been published & validated for use in WB.

Qualität

Routinely evaluated by immunoblot.

Zielbeschreibung

180 kDa

Physikalische Form

Thiophilic adsoption and size exclusion chromatography
100 µg of mouse monoclonal IgG1 liquid in 0.01 mg/ml PBS containing 0.09% sodium azide, PEG, and sucrose with 50 % glycerol.
Format: Purified

Lagerung und Haltbarkeit

6 months at -20°C from date of shipment

Hinweis zur Analyse

Control
Includes PMA and pervanadate treated A431 cell lysate as a positive control

Rechtliche Hinweise

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

10 - Combustible liquids

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Die Dokumentenbibliothek aufrufen

Cisplatin induces PKB/Akt activation and p38(MAPK) phosphorylation of the EGF receptor
Winograd-Katz, S E and Levitzki, A
Oncogene, 25, 7381-7390 (2006)
Mutational removal of the Thr669 and Ser671 phosphorylation sites alters substrate specificity and ligand-induced internalization of the epidermal growth factor receptor
Heisermann, G J, et al
The Journal of Biological Chemistry, 265, 12820-12827 (1990)
G J Heisermann et al.
The Journal of biological chemistry, 263(26), 13152-13158 (1988-09-15)
The epidermal growth factor (EGF) receptor is regulated by EGF-stimulated autophosphorylation and by phorbol ester-stimulated, protein kinase C (Ca2+/phospholipid-dependent enzyme) mediated phosphorylation at identified sites. The EGF receptor contains additional phosphorylation sites including a prominent phosphothreonine and several phosphoserines which
F Yan et al.
Oncogene, 36(50), 6919-6928 (2017-09-05)
Overexpression of DNMT1 and KIT is prevalent in lung cancer, yet the underlying molecular mechanisms are poorly understood. While the deregulated activation of DNMT1 or KIT has been implicated in lung cancer pathogenesis, whether and how DNMT1 and KIT orchestrate

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