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HPLC Analysis of Aminopterin and Related Compounds on Ascentis® Express C18

HPLC Analysis of Aminopterin and Related Compounds on Ascentis® Express C18 application for HPLC

Materiales

analytical column

Referencia del producto
Descripción
Precios

53829-U

Columna para HPLC Ascentis® Express C18, 2,7 μm

2.7 μm particle size, L × I.D. 15 cm × 4.6 mm

standard

Referencia del producto
Descripción
Precios

F7876

Folic acid

≥97%

M9929

Metotrexato hydrate

meets USP testing specifications

CONDITIONS

column

Ascentis Express C18, 15 cm x 4.6 mm I.D., 2.7 μm particles (53829-U)

mobile phase

[A] 10 mM ammonium formate, pH 3.0; [B] acetonitrile; (90:10, A:B)

flow rate

1.0 mL/min

column temp.

35 °C

detector

UV, 280 nm

injection

5 μL

Descripción

Analysis Note

This application demonstrates the suitability of the Ascentis C18 for the analysis of aminopterin, folic acid and methotrexate. Structures along with the optimized chromatogram obtained on the Ascentis Express C18 are presented

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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