843153

TOYOPEARL^® hydrophobic interaction media

phase C4 (butyl), bottle of 25 mL, 35 μm particle size

• Sinónimos: TOYOPEARL^® Butyl-650 HIC Bulk Media
• UNSPSC Code: 41115700

Propiedades

• description: Butyl-650S
• product line: TOYOPEARL^®
• form: slurry
• packaging: bottle of 25 mL
• manufacturer/tradename: Tosoh 43153
• parameter: 3 bar max. pressure
• technique(s): LPLC: suitable
• matrix: hydroxylated methacrylic polymer
• matrix active group: C4 (butyl) phase
• particle size: 35 μm
• operating pH: 1-13
• capacity: 40 g/L±10 adsorption capacity (lysozyme)
• separation technique: hydrophobic interaction (HIC)

Descripción

General description

El medio Toyopearl para (HIC) ofrece las siguientes ventajas:
¿ Fuerte afinidad para proteínas solubles en agua.
¿ Alta recuperación de masa y actividad
¿ Alta capacidad de muestra - 2-4 veces superior a la de geles convencionales
¿ Las concentraciones fluctuantes de sales no cambiaran el volumen de cama
¿ Estabilidad mecánica de 7kg/cm2 (7bar/100psi)
¿ Rango de pH 2-12 ? Se lava en el lugar con O.5M NaOH
¿ Autoclavable
La media Toyopearl ofrece grandes cantidades en recuperación de proteínas usando varios eluyentes acuosos. Las resinas tienen un límite de exlusión de 5 x 10e6 Daltons. Los grandes tamaños en los poros, 1000Å, le permite a estos empaques separar proteínas muy grandes por mecanismos de interacción hidrofóbica, sin los efectos de exclusión por tamaño.
Botella 100mL

TOYOPEARL^® HIC resins are hydrophobic interaction chromatography resins that offer the following advantages: strong affinity for water-soluble proteins, high recovery of mass and activity, high sample capacity up to 2-4 times that of gel media, fluctuating salt concentrations will not change the bed volume, mechanical stability to 7kg/cm squared (7 bar/100psi), stability at a wide pH range (2-12), clean in place with 0.5M NaOH, and autoclavable. The exclusion limit of 5x10⁶ Da and large pore size, 1000Å, enables these packings to separate very large proteins by a hydrophobic interaction mechanism, without size exclusion effects.

Specifications

Clean in place with 0.5 M NaOH or 0.1 M HCl.

Physical form

72% (v/v) slurry in 20% (v/v) ethanol

Other Notes

Toyopearl Butyl-650 resin has the second highest hydrophobicity of the HIC ligands offered by TBL. Its pore size is the largest of our three butyl resins. Primary applications are for the separation of proteins, their isoforms, and aggregate removal. It is typically used in interresinte purification and polishing steps

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

Toyopearl is a registered trademark of Tosoh Corporation

Información de seguridad

• pictograms: GHS02
• signalword: Warning
• hcodes: H226
• pcodes: P210 - P233 - P240 - P241 - P242 - P243
• Hazard Classifications: Flam. Liq. 3
• Storage Class: 3 - Flammable liquids
• wgk_germany: WGK 3
• flash_point_f: 104.0 °F - closed cup
• flash_point_c: 40 °C - closed cup