ZRB1836
Anti-Histone H3-Lys9me2 Antibody, clone 2J13 ZooMAb® Rabbit Monoclonal
recombinant, expressed in HEK 293 cells
Sinónimos:
H3K9me2;Histone H3-Lys9me2
About This Item
biological source
rabbit
Quality Level
recombinant
expressed in HEK 293 cells
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
2J13, recombinant monoclonal
description
2J13 Clone
product line
ZooMAb® learn more
form
lyophilized
mol wt
calculated mol wt 15.4 kDa
observed mol wt ~16 kDa
purified by
using Protein A
species reactivity
human, rat
packaging
antibody small pack of 25 μL
greener alternative product characteristics
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
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enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable
inhibition assay: suitable
western blot: suitable
isotype
IgG
epitope sequence
N-terminal
Protein ID accession no.
UniProt accession no.
greener alternative category
, Aligned
shipped in
ambient
storage temp.
2-8°C
target post-translational modification
dimethylation (Lys9)
General description
Specificity
Immunogen
Application
Evaluated by Western Blotting in C6 cell lysate.
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected Di-Methyl-Histone H3-Lys9 (H3K9me2) in C6 cell lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Histone H3-Lys9me2 in acid extracts of HL-60 and HeLa cells.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Histone H3-Lys9me2 in C6 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Histone H3-Lys9me2 in human liver tissue sections.
Peptide Inhibition Assay: Target band detection in acid extract of HeLa cells was prevented by pre-blocking of a representative lot with di-methylated Histone H3-Lys9 peptide, but not the corresponding non-methylated Histone H3-Lys9 peptide.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Target description
Physical form
Reconstitution
Storage and Stability
Legal Information
Disclaimer
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Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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