ZMS1194

Anti-PCNA Antibody, clone PC10 ZooMAb^® Mouse Monoclonal

recombinant, expressed in HEK 293 cells

• Sinónimos: Proliferating cell nuclear antigen
• UNSPSC Code: 12352203

Propiedades

• biological source: mouse
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: PC10, recombinant monoclonal
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 28.77 kDa; observed mol wt ~33 kDa
• purified by: using protein G
• species reactivity: mouse, rat, human
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• technique(s): immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG2aκ
• epitope sequence: Unknown
• Protein ID accession no.: NP_071776.1
• UniProt accession no.: P04961
• greener alternative category: Aligned
• shipped in: ambient
• storage temp.: 2-8°C
• Gene Information: rat ... PCNA(25737)

Descripción

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry. This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone PC10 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects PCNA.

Immunogen

Full-length, recombinant rat PCNA.

Application

Quality Control Testing

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected PCNA in HeLa cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected PCNA in C2C12 and C6 cell lysates.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected PCNA in Human tonsil tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected PCNA in HeLa cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Proliferating cell nuclear antigen (UniProt: P04961; also known as PCNA) is encoded by the PCN gene (Gene ID: 25737) in rat. PCNA is a is an evolutionarily conserved protein found in all eukaryotic species. It is a ring-shaped homotrimeric nuclear protein that serves as an auxiliary protein of DNA polymerase delta and epsilon and is involved in the control of eukaryotic DNA replication by increasing the polymerase′s processability during elongation of the leading strand. Its maximum expression is observed during the S phase of the cell cycle. It Induces a robust stimulatory effect on the 3′-5′ exonuclease and 3′-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. It is reported to colocalize with CREBBP, EP300 and POLD1 to sites of DNA damage. It acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote post-replication repair. PCNA can undergo phosphorylation on tyrosine 211 by EGFR that stabilizes chromatin-associated PCNA. Following DNA damage, it can undergo monoubiquitination to stimulate direct bypass of DNA lesions by specialized DNA polymerases or polyubiquitination to promote recombination-dependent DNA synthesis across DNA lesions by template switching mechanisms.  Mutations in PCN gene are known to cause Ataxia-telangiectasia-like disorder 2 (ATLD2) that is characterized by developmental delay, ataxia, hearing loss, and short stature. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Gonzalez-Magana, A., and Blanco, FJ.(2020). Biomolecules. 10(4); 570; Strzalka, W., and Ziemienowicz, A. (2011). Ann. Bot. 107(7); 1127-1140).

Physical form

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

30 µg/mL mg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable