Y1771

Yeast Synthetic Drop-out Medium Supplements

without uracil, leucine, and tryptophan

• Sinónimos: Synthetic yeast drop-out mix, Yeast Synthetic Drop-out Media Supplements
• MDL number: MFCD01095679
• UNSPSC Code: 41106212
• NACRES: NA.85

Propiedades

• Quality Level: 200
• form: powder
• technique(s): transformation: suitable
• application(s): microbiology
• storage temp.: room temp

Descripción

Application

Yeast Synthetic Drop-out Medium Supplements, without uracil, leucine, and tryptophan has been used for screening:

• the transformants of the PO1f strains which carried the pCRISPRyl_URA3sgRNA plasmids
• Yarrowia lipolytica PO1f strain positive transformants
• CRISPR- and RNA-assisted in vivo directed evolution (CRAIDE) mutants

The selection of plasmids in yeast is based on the use of auxotrophic mutant strains that cannot grow without a specific media component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. Sigma′s Yeast Synthetic Drop-Out Media Supplements create a richer medium for better yield and growth rate, and increase the probability of successful transformations when screening libraries or performing gene knock-outs.

Other Notes

Mixtures of amino acids and other nutrients to be added to Yeast Nitrogen Base Without Amino Acids.

Preparation Note

Dissolve 1.46 g of supplement per liter of medium.

Información de seguridad

• pictograms: GHS05
• signalword: Danger
• hcodes: H318
• pcodes: P280 - P305 + P351 + P338
• Hazard Classifications: Eye Dam. 1
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable