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Merck

T7684

Sigma-Aldrich

Transaminase, broad-range bacterial

recombinant, expressed in E. coli, lyophilized powder, ≥5 units/mg solid

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About This Item

MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

recombinant

expressed in E. coli

Quality Level

form

lyophilized powder

specific activity

≥5 units/mg solid

storage temp.

2-8°C

General description

Increased transaminase activity alters the rate at which precursors of protein are interchanged with carbohydrate intermediates.

Application

Transaminase has been used in a study to assess the effect of ischemic preconditioning on pathophysiologic alterations of hemodynamic, biochemical and inflammatory parameters observed during intra-abdominal hypertension. Transaminase levels have also been used in a study to investigate the use of hydrogen-1 MR spectroscopy for measurement and diagnosis of hepatic steatosis.

Unit Definition

One unit will produce 1.0 μmole of oxaloacetate from L-aspartate during the transamination of 2-ketoglutarate per min at pH 7.0 at 25 °C in the presence of pyridoxal phosphate.

Physical form

Lyophilized powder containing potassium phosphate buffer salts

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Alexandra Avraamidou et al.
Langenbeck's archives of surgery, 397(8), 1333-1341 (2012-07-05)
Intra-abdominal hypertension (IAH) has several pathophysiologic implications on human organs and systems. The aim of this experimental study was to investigate whether ischemic preconditioning (IP), namely the application of IAH for a small period of time prior to establish pneumoperitoneum
Glucocorticosteroids and transaminase activity. I. Increased activity of glutamicpyruvic transaminase in four conditions associated with gluconeogenesis.
F ROSEN et al.
The Journal of biological chemistry, 234(3), 476-480 (1959-03-01)
Patrick Georgoff et al.
AJR. American journal of roentgenology, 199(1), 2-7 (2012-06-27)
Hydrogen-1 MR spectroscopy ((1)H-MRS) is gaining acceptance as a noninvasive technique for assessment of hepatic steatosis, and the findings have been found to correlate closely with histopathologic grade. The aims of this study were to validate (1)H-MRS performed with a

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