T5319
Tetanolysin from Clostridium tetani
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About This Item
biological source
Clostridium tetani E88
mol wt
55 kDa
application(s)
cell analysis
storage temp.
2-8°C
Gene Information
Clostridium tetani E88 ... CTC01888(1060232)
General description
Hemolytic activity of tetanolysin is determined using 2.5% rabbit red blood cells at 37 °C for 40 min.
Application
Tetanolysin has been used to analyze the formation of lytic pores in red blood cells (RBCs)1. It has also been used to permeabilize infected RBCs2.
Biochem/physiol Actions
Cholesterol-binding toxin used to permeabilize cellular membranes to enhance the entry of macromolecules into the interior of the cell. Pores induced reported to be in the range of 20-50 nm.
Preparation Note
Prepared by a modification of the method of Haque, et al.
Reconstitution
When reconstituted with 100 μl of sterile water the concentration is 1 μg/μl in 40 mM sodium phosphate buffer, pH 7.2, containing 200 mM NaCl.
Analysis Note
Single band by SDS-PAGE.
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Certificados de análisis (COA)
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Hayley E Bullen et al.
The Journal of biological chemistry, 287(11), 7871-7884 (2012-01-19)
To survive within its host erythrocyte, Plasmodium falciparum must export hundreds of proteins across both its parasite plasma membrane and surrounding parasitophorous vacuole membrane, most of which are likely to use a protein complex known as PTEX (Plasmodium translocon of
Jason W Rosch et al.
The Journal of clinical investigation, 120(2), 627-635 (2010-01-23)
Sickle cell disease (SCD) is characterized by intravascular hemolysis and inflammation coupled to a 400-fold greater incidence of invasive pneumococcal infection resulting in fulminant, lethal pneumococcal sepsis. Mechanistically, invasive infection is facilitated by a proinflammatory state that enhances receptor-mediated endocytosis
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