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Key Documents

SRP3312

Sigma-Aldrich

IL-10 human

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)

Sinónimos:

B-TCGF, CSIF, TGIF

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.32

biological source

human

recombinant

expressed in E. coli

assay

≥98% (HPLC)
≥98% (SDS-PAGE)

form

lyophilized

potency

≤2.0 ng/mL

mol wt

18.6 kDa

packaging

pkg of 10 μg

impurities

endotoxin, tested

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... IL10(3586)

General description

Interleukin-10 (IL-10) is an immunosuppressive cytokine produced by a variety of mammalian cell types including macrophages, monocytes, T cells, B cells and keratinocytes. Human IL-10 active on murine cells, but murine IL-10 is inactive on human cells. The gene encoding this protein is localized on human chromosome 1q31-32. Recombinant human IL-10 is an 18.6 kDa protein of 161 amino acid residues. Interleukin 10 (IL-10) comprises interferon IFN-γ fold and exists as a symmetric homodimer. It shares structural similarity with interferon γ (IFN-γ).

Application

Interleukin 10 (IL-10) human has been used in enzyme-linked immunosorbent assay.

Biochem/physiol Actions

Interleukin-10 (IL-10) is an anti-inflammatory protein that inhibits the expression of proinflammatory cytokines such as IL-1 and tumor necrosis factor-α (TNF-α). Like IL-4, IL-10 enhances humoral immune responses and attenuates cell-mediated immune reactions. It inhibits the production of IFN-γ and antigen-specific proliferation of type-1 T helper cells (Th1). It protects the host tissue from damage during immune responses. The protein has a role in modulating viral immunity. IL-10 enhances cytokine production, natural killer cell proliferation and cytotoxicity in vitro. IL-10 is an immunosuppressive cytokine. It promotes wound repair and functions to resolve inflammation in the central nervous system. IL-10 mediates its signaling function through the interleukin receptors namely, IL-10RA and two IL-10RBs. The expression of IL-10 is regulated by signal transducer and activator of transcription 3 (STAT3) and suppressor of cytokine signaling-3 (SOCS3) ratios. Polymorphisms in the IL-10 gene is implicated in breast cancer.

Physical form

Lyophilized from 20 mM Sodium Phosphate, pH 7.2.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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IL-10: A Multifunctional Cytokine in Viral Infections
Jose M. Rojas
Journal of immunology research (2017)
Infection of human macrophages and Dendritic Cells with Mycobacterium tuberculosis Induces a Differential Cytokine Gene Expression That Modulates T Cell response
Journal of Immunology (2001)
Amanda R Burmeister et al.
Frontiers in cellular neuroscience, 12, 458-458 (2018-12-14)
Resident cells of the central nervous system (CNS) play an important role in detecting insults and initiating protective or sometimes detrimental host immunity. At peripheral sites, immune responses follow a biphasic course with the rapid, but transient, production of inflammatory
Relationship between IL-10 gene -819C/T polymorphism and the risk of inflammatory bowel disease: a meta-analysis
Haien Wu
African Health Sciences, 16(3), 866-872 (2016)
P T Mehrotra et al.
Journal of immunology (Baltimore, Md. : 1950), 160(6), 2637-2644 (1998-03-24)
Human NK cell activity can be augmented in vitro by stimulation with IL-2 or IL-12, both of which also induce the production of IFN-gamma, TNF-alpha, and granulocyte-macrophage CSF by NK cells. For the first time, we demonstrate that freshly purified

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