SRE0053

Lisostafina from Staphylococcus staphylolyticus

Suitable for manufacturing of diagnostic kits and reagents., lyophilized powder

• Sinónimos: Endopeptidasa glicil-glicina
• Número de CAS: 9011-93-2
• Comisión internacional de enzimas: 3.4.24.75 (BRENDA, IUBMB)
• MDL number: MFCD00131556
• UNSPSC Code: 12352204
• NACRES: NA.32

Propiedades

• biological source: bacterial (Staphylococcus sp.)
• Quality Level: 400
• form: lyophilized powder
• specific activity: ≥500 units/mg protein
• mol wt: 26,926 Da
• composition: protein, 50-70% biuret
• packaging: pkg of (package size sold on basis of mg protein)
• concentration: 50—70% protein
• suitability: suitable for microbiological tests
• application(s): diagnostic assay manufacturing
• shipped in: wet ice
• storage temp.: −20°C

Descripción

General description

Lysostaphin is a glycylglycine endopeptidase. It is an important antibacterial enzyme.

Biochem/physiol Actions

La lisostafina es una endopeptidasa de cinc con un peso molecular de aproximadamente 25 kDa. Debido a que la lisostafina rompe los enlaces transversales de la poliglicina de la capa de peptidoglucanos de la pared celular de las especies de Staphylococcus , se ha encontrado útil para la lisis celular y también como potencial tratamiento antimicrobiano.
pH de actividad óptimo: ~7,5

Lysostaphin has the ability to cleave both viable and heat-killed S. aureus cells. It is one of the major antibacterial agents for the staphylococci. Lysostaphin cleaves the cross-linking pentaglycine bridges in the cell walls of staphylococci. Thus it is considered as an antistaphylococcal therapeutic agent.

Unit Definition

One unit will reduce the turbidity (A₆₂₀) of a suspension of Staphylococcus aureus cells from 0.250 to 0.125 in 10 min at pH7.5 at 37°C in a 6.0ml reaction mixture.

Información de seguridad

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable