SCT089
BioTracker™ rRNA Live Cell Probe
Fluorescent detection of ribosomal RNA and cell nucleoli
Sinónimos:
live cell probe, nucleoli probe, nucleolus probe, rRNA dye, rRNA probe, ribosomal RNA probe
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About This Item
UNSPSC Code:
12171501
NACRES:
NA.31
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General description
The BioTracker™rRNA live cell probe is a styryl quinolinium dye that selectively binds ribosomal RNA (rRNA) in the cytoplasm and nucleoli of mammalian cells 1. This probe is a fluorescent small molecule that demonstrates high affinity to rRNA and therefore enables imaging of the nucleolus. Due to its low cytotoxicity, it enables detection in living systems with minimal interference.1 . As imaging technologies progress, there is increased demand for reagents that can detect diverse organoids and enable observation of subcellular functions in living cells. The optimal fluorescent reagent for live cell imaging should show high specificity to target along with low cytotoxicity 2 . Because the nucleolus is a dense organoid composed of chromatin and numerous protein components, the development of fluorescent reagents for live cell nucleoli detection is even more challenging 2,3,4. BioTracker™ rRNA live cell probe is easy to use, emits light in a longer wavelength to reduce phototoxicity and facilitate multiplexing, and can enhance nucleolar imaging dynamics.
Spectral Properties:Fluorescence images obtained by λex = 570 nm and emission at 647-667 nm.
Spectral Properties:Fluorescence images obtained by λex = 570 nm and emission at 647-667 nm.
Application
Live cell imaging probefor detection of ribosomal RNA (rRNA). Can also be used in fixed cell or tissuesamples, such as with a nuclear counterstain to identify nucleoli.
Quality
Purity: ≥ 95% confirmed by HPLC. Molar Mass: 298 Da confirmed byLC-MS.
Storage and Stability
Store BioTracker™ rRNA Live CellProbe at 2-8 °C, desiccated and protected from light
Note: Centrifuge vial briefly to collect contents at bottom ofvial before opening.
Note: Centrifuge vial briefly to collect contents at bottom ofvial before opening.
Legal Information
BioTracker is a trademark of Merck KGaA, Darmstadt, Germany
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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