SAE0196
Achromopeptidase from bacteria
free of DNA contaminants, suitable for Microbiome research
Sinónimos:
Bacterial Protease
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About This Item
UNSPSC Code:
12352204
NACRES:
NA.54
Productos recomendados
biological source
bacterial
Quality Level
form
lyophilized powder
specific activity
≥1000 U/mg
feature
DNA free
technique(s)
DNA extraction: suitable
suitability
suitable for cell lysis
application(s)
cell analysis
shipped in
ambient
storage temp.
−20°C
General description
Achromopeptidase is useful for lysis of Gram-positive bacteria that are resistant to lysozyme, as well as Gram-negative organisms. Achromopeptidase has been shown to contain two bacteriolytic proteases. This product is free of detectable microbial DNA and is suitable for microbiome research
Application
Achromopeptidase is useful for lysis of Gram-positive bacteria that are resistant to lysozyme, as well as Gram-negative organisms. Achromopeptidase has been shown to contain two bacteriolytic proteases.
The study of microbial communities has been revolutionized in recent years by the widespread adoption of culture independent analytical techniques such as 16S rRNA gene sequencing and metagenomics. Since DNA contamination during sample preparation is a major problem of these sequence-based approaches, DNA extraction reagents free of DNA contaminants are essential. This product undergoes strict quality control testing to ensure the absence of detectable levels of contaminating microbial DNA using 35 cycles PCR amplification of 16S and 18S rDNA using universal primer sets.
The study of microbial communities has been revolutionized in recent years by the widespread adoption of culture independent analytical techniques such as 16S rRNA gene sequencing and metagenomics. Since DNA contamination during sample preparation is a major problem of these sequence-based approaches, DNA extraction reagents free of DNA contaminants are essential. This product undergoes strict quality control testing to ensure the absence of detectable levels of contaminating microbial DNA using 35 cycles PCR amplification of 16S and 18S rDNA using universal primer sets.
Biochem/physiol Actions
Achromopeptidase is a lysyl endopeptidase that has a molecular weight of approximately 27 kDa. Optimum pH is 8.5-9.
Features and Benefits
Contains two bacteriolytic proteases for efficient lysis of Gram-positive bacteria and Gram-negative organisms
Free of detectable microbial DNA, ensuring the accuracy of metagenomics and other culture-independent sequencing methods
Undergoes strict quality control testing to ensure the absence of detectable levels of contaminating microbial DNA
Optimum pH of 8.5-9 allows for use in a wide range of microbiome studies
Free of detectable microbial DNA, ensuring the accuracy of metagenomics and other culture-independent sequencing methods
Undergoes strict quality control testing to ensure the absence of detectable levels of contaminating microbial DNA
Optimum pH of 8.5-9 allows for use in a wide range of microbiome studies
Unit Definition
One unit will lyse 0.4 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 8.0 at 37 °C.
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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