SAB4200248
Anti-Actin antibody, Mouse monoclonal
clone MM2/193, purified from hybridoma cell culture
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About This Item
conjugate:
unconjugated
application:
WB
clone:
MM2/193, monoclonal
species reactivity:
mouse, human, rat, canine, monkey
citations:
13
technique(s):
western blot: 1-2 μg/mL using HeLa cell extracts
biological source
mouse
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
MM2/193, monoclonal
form
buffered aqueous solution
mol wt
antigen ~42 kDa
species reactivity
mouse, human, rat, canine, monkey
concentration
~1.0 mg/mL
technique(s)
western blot: 1-2 μg/mL using HeLa cell extracts
isotype
IgM
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... ACTB(60)
General description
Monoclonal anti-actin (mouse IgM isotype) is derived from the hybridoma MM2/193 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with human platelet membranes. Monoclonal anti-actin recognizes human, monkey, dog, rat and mouse actin (not tested in other species).
In mammals, actin protein consists of six different isoforms encoded by separate genes. The four isoforms αskeletal-actin, α cardiac-actin, αsmooth-actin and γsmooth-actin are expressed in skeletal, cardiac and smooth muscle. The other two isoforms, β cyto-actin and γ cyto-actin are ubiquitously expressed.
In mammals, actin protein consists of six different isoforms encoded by separate genes. The four isoforms αskeletal-actin, α cardiac-actin, αsmooth-actin and γsmooth-actin are expressed in skeletal, cardiac and smooth muscle. The other two isoforms, β cyto-actin and γ cyto-actin are ubiquitously expressed.
Immunogen
hybridoma MM2/193 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with human platelet membranes
Application
Monoclonal Anti-Actin antibody produced in mouse has been used in immunoblotting.
Biochem/physiol Actions
Actin plays a vital role in various cellular processes, such as cell division, migration, junction formation, chromatin remodeling, transcriptional regulation, vesicle trafficking and cell shape regulation. Polymorphism in the smooth muscle alpha-actin (ACTA2) is associated with the development of thoracic aortic aneurysms and dissections.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide
Storage and Stability
Store at -20°C. For continuous use, store at 2-8°C for up to one month. For extended storage, freeze at -20°C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Other Notes
In order to obtain the best results using various techniques and preparations, we recommend determining optimal working dilutions by titration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Referencia del producto
Descripción
Precios
Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Mutations in smooth muscle alpha-actin (ACTA2) lead to thoracic aortic aneurysms and dissections.
Guo DC, et al.
Nature Genetics, 39, 1488-1493 (2007)
Jie Zhou et al.
Molecular medicine reports, 15(6), 4123-4131 (2017-05-11)
Total resection of adamantinomatous craniopharyngioma (ACP) is complex and often leads to postoperative recurrence. This is due to the tendency of the tumor to invade the surrounding brain tissue and the generation of a local inflammatory state between the tumor
Kun Zhu et al.
European journal of medical research, 27(1), 5-5 (2022-01-14)
Intervertebral disc degeneration (IDD) is a natural progression of age-related processes. Associated with IDD, degenerative disc disease (DDD) is a pathologic condition implicated as a major cause of chronic lower back pain, which can have a severe impact on the
Insulin increases filtration barrier permeability via TRPC6-dependent activation of PKGIa signaling pathways.
Rogacka D, et al.
Biochimica et Biophysica Acta, 1863, 1312-1325 (2017)
The Actin Gene Family: Function Follows Isoform
Perrin BJ and Ervasti JM
Cytoskeleton (Hoboken, N.J.), 67, 630-634 (2010)
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
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