SAB1100050

Anti-LDHA (216-228) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• Sinónimos: Anti-LDH-M, Anti-LDH1, Anti-Lactate dehydrogenase A, Anti-PIG19
• UNSPSC Code: 12352203
• NACRES: NA.41

Propiedades

• biological source: rabbit
• conjugate: unconjugated
• antibody form: IgG fraction of antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~37 kDa
• species reactivity: human
• technique(s): western blot: 1:500-1:2,000
• UniProt accession no.: P00338
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... LDHA(3939)

Descripción

General description

Lactate dehydrogenase A (LDHA) or the M subunit is a cytosolic enzyme and is abundantly present in the skeletal muscle. Lactate dehydrogenase B (LDHB) or the H subunit is mainly seen in the heart. Both genes are capable of forming homotetramers or heterotetramers. LDHA gene is located on human chromosome 11p15.4.

Immunogen

synthetic peptide corresponding to amino acids 216-228 of human LDHA

Application

Anti-LDHA (216-228) antibody produced in rabbit has been used in:

• in situ

• proximity ligation assay (PLA)
• immuno-staining
• western blotting

Yale Center for High Throughput Cell Biology IF-tested antibodies. Each antibody is tested by immunofluorescence against HUVEC cells using the Yale HTCB IF protocol. To learn more about us and Yale Center for High Throughput Cell Biology partnership, visit sigma.com/htcb-if.

Biochem/physiol Actions

Lactate dehydrogenase A (LDHA) participates in anaerobic and aerobic glycolysis. It can regenerate nicotinamide adenine dinucleotide (NAD⁺) from NADH to maintain the ATP synthesis in the absence of adequate oxygen. The N-terminus of LDHA is essential for the stability of the structure.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable