RAB0149
Human EGF ELISA Kit
for serum, plasma, cell culture supernatant.
Sinónimos:
EGF ELISA Kit
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About This Item
UNSPSC Code:
41116158
NACRES:
NA.32
Productos recomendados
species reactivity
human
packaging
kit of 96 wells (12 strips x 8 wells)
technique(s)
ELISA: suitable
capture ELISA: suitable
input
sample type cell culture supernatant(s)
sample type plasma
sample type serum
assay range
inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 1 pg/mL
standard curve range: 0.82-200 pg/mL
detection method
colorimetric
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... EGF(1950)
General description
The Human EGF ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human EGF in serum, plasma and cell culture supernatants.
Immunogen
Recombinant Human EGF
Application
For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Human EGF ELISA Kit has been used to examine the concentration of epidermal growth factor (EGF) in adipose derived stem cell conditioned medium (ADSC-CM).
Biochem/physiol Actions
Epidermal growth factor (EGF) is the member of EGF-like family of growth factors, which binds to EGF receptor. EGF increases proliferation and migration in bone marrow stromal cells (BMSC), that gives rise to osteoblasts, chondrocytes and adipocytes. It blocks gastric acid and pepsin secretion and enhances gastrointestinal cell proliferation. It functions as a strong mitogenic factor for various cultured cells. Mutations in this gene is associated with hypomagnesemia..
Other Notes
A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.
Please type the word sample in the text box provided for lot number.
Los componentes del kit también están disponibles por separado
Referencia del producto
Descripción
SDS
signalword
Warning
hcodes
pcodes
Hazard Classifications
Met. Corr. 1
Storage Class
8A - Combustible corrosive hazardous materials
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Medical Journal of Indonesia, 26(4), 239-245 (2018)
Human epidermal growth factor precursor: cDNA sequence, expression in vitro and gene organization
Bell GI, et al.
Nucleic Acids Research, 14(21), 8427-8446 (1986)
Impaired basolateral sorting of pro-EGF causes isolated recessive renal hypomagnesemia
Groenestege WMT, et al.
The Journal of Clinical Investigation, 117(8), 2260-2267 (2007)
Epidermal growth factor as a mechanosensitizer in human bone marrow stromal cells
Muller-Deubert S, et al.
Stem Cell Research, 24, 69-76 (2017)
Lijun Wang et al.
American journal of physiology. Cell physiology, 307(8), C710-C717 (2014-08-08)
We used primary mouse corneal epithelial cells (pMCE) to examine the role of Kcnj10 in determining membrane K(+) conductance and cell membrane potential and in regulating EGF/TGFA release. Western blot, immunostaining, and RT-PCR detected the expression of Kcnj10 in mouse
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