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P8172

Sigma-Aldrich

Anti-Rabbit IgG (whole molecule), F(ab′)2 fragment−R-Phycoerythrin antibody produced in goat

affinity isolated antibody, buffered aqueous solution

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About This Item

MDL number:
MFCD00162789
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

goat

Quality Level

200

conjugate

phycoerythrin (R-PE) conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

technique(s)

indirect immunofluorescence: 1:20

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

General description

Immunoglobulins (Igs) are glycoprotein antibodies that modulate several immune responses. Rabbit IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rabbit IgGs can be useful tools for the analysis of target proteins
Anti-Rabbit IgG (whole molecule), F(ab′)2 fragment-R-Phycoerythrin antibody detects all rabbit immunoglobulins.

Immunogen

Purified Rabbit IgG

Application

Anti-Rabbit IgG (whole molecule), F(ab′)2 fragment-R-Phycoerythrin antibody is suitable for use in flow cytometry and immunofluorescence.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Flow cytometry/Cell sorting (1 paper)

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.1 mM EDTA, 1 mM iodoacetamide, 1% bovine serum albumin and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Martin Cheung et al.
Molecular microbiology, 95(1), 31-50 (2014-10-30)
Type III secretion systems are found in many Gram-negative bacteria. They are activated by contact with eukaryotic cells and inject virulence proteins inside them. Host cell detection requires a protein complex located at the tip of the device's external injection
M Brandes et al.
International immunology, 12(9), 1285-1292 (2000-09-01)
In this study we have examined the migration responses of human peripheral blood or tonsillar B lymphocytes to a selection of 27 chemokines. Freshly isolated (CD19(+)) B lymphocytes show greatly impaired in vitro chemotaxis which is overcome by overnight culture.
Graham P Feeney et al.
Haematologica, 90(2), 180-187 (2005-02-16)
The HFE protein interacts with the transferrin receptor (TfR) to regulate cellular iron uptake. Nucleated erythroid cells have the highest number of TfR and the greatest iron uptake. The aim of this study was to investigate whether erythroid iron uptake

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