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P7962

Sigma-Aldrich

Monoclonal Anti-PSTAIR antibody produced in mouse

clone PSTAIR, ascites fluid

Sinónimos:

Anti-CDC2

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About This Item

MDL number:
MFCD00677382
UNSPSC Code:
12352203
NACRES:
NA.43

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

PSTAIR, monoclonal

mol wt

antigen 33-34 kDa

contains

15 mM sodium azide

species reactivity

mouse, Japanese quail, human, amago salmon, eel, starfish, lily, carp, onion, goldfish, monkey, Xenopus, ciliates

technique(s)

immunoprecipitation (IP): suitable
indirect ELISA: suitable
western blot: 1:4,000 using a COS-7 cell extract

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CDC2(983)
mouse ... Cdc2a(12534)

Categorías relacionadas

General description

In every eukaryote examined, CDKs contain an evolutionary conserved 16 amino acid sequence called PSTAIR (EGVPSTAIREISLLKE) which distinguishes them from other protein kinases. The PSTAIR motif is involved in complex formation with cyclins. The availability of monoclonal antibody reacting specifically with the PSTAIR sequence enables the subcellular detection and localization of the various CDKs and examination of substrate interactions, in a variety of organisms.
Monoclonal Anti-PSTAIR (mouse IgG1 isotype) is derived from the PSTAIR hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Cyclin-dependent kinase 1 (CDC2/CDK1) belongs to the Ser/Thr protein kinase family. It is located on human chromosome 10q21.

Immunogen

synthetic 16 amino acid oligopeptide containing the PSTAIR sequence conjugated to BSA.

Application

Monoclonal Anti-PSTAIR antibody produced in mouse has been used in:
  • western blot analysis
  • enzyme-linked immunosorbent assay (ELISA)
  • immunoprecipitation

Mouse monoclonal clone PSTAIR anti-PSTAIR antibody may be used for the localization of cyclin-dependent kinases containing the PSTAIR motif using various immunochemical assays including ELISA, immunoblot and immunoprecipitation.

Biochem/physiol Actions

Cyclin-dependent kinase 1 (CDC2/CDK1) controls various mitotic processes including the reorganization of the cytoskeleton, chromosome segregation, and formation and separation of daughter cells. It is essential for the multiplication of mammalian cell. It participates in tumorigenesis.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Descripción
Precios

Storage Class

10 - Combustible liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Martin Převorovský et al.
PloS one, 10(9), e0137820-e0137820 (2015-09-15)
Cbf11 and Cbf12, the fission yeast CSL transcription factors, have been implicated in the regulation of cell-cycle progression, but no specific roles have been described and their target genes have been only partially mapped. Using a combination of transcriptome profiling
Héloïse Chassé et al.
PloS one, 11(3), e0150318-e0150318 (2016-03-11)
The cyclin B/CDK1 complex is a key regulator of mitotic entry. Using PP242, a specific ATP-competitive inhibitor of mTOR kinase, we provide evidence that the mTOR signalling pathway controls cyclin B mRNA translation following fertilization in Sphaerechinus granularis and Paracentrotus
Masaki Ishikawa et al.
The Plant cell, 23(8), 2924-2938 (2011-08-25)
During regeneration, differentiated plant cells can be reprogrammed to produce stem cells, a process that requires coordination of cell cycle reactivation with acquisition of other cellular characteristics. However, the factors that coordinate the two functions during reprogramming have not been
Casein kinase 2 regulates telomere protein complex formation through Rap1 phosphorylation
Inoue H, et al.
Nucleic Acids Research (2019)
Gui Ying Li et al.
Cell research, 14(2), 169-175 (2004-04-30)
CDKs play key roles in controlling cell cycle progression in all eukaryotes. In plants, multiple CDKs are present, among which the best characterized CDKs are PSTAIRE CDKs. In this study, we carried out Western blot, immunoelectron microscopy and antibody treatment
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