Saltar al contenido
Merck
Todas las fotos(2)

Key Documents

View All Documentation

P4664

Sigma-Aldrich

o-Fenilendiamina dihydrochloride

chromogenic, tablet

Sinónimos:

1,2-Phenylenediamine tablet, OPD, OPD Tablet

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
Todas las fotos(2)

About This Item

Fórmula lineal:
C6H4(NH2)2·2HCl
Número de CAS:
615-28-1
Peso molecular:
181.06
Beilstein/REAXYS Number:
3912045
MDL number:
MFCD00012966
UNSPSC Code:
12352204
PubChem Substance ID:
24898491
NACRES:
NA.83

product name

o-Fenilendiamina dihydrochloride, tablet, 15 mg substrate per tablet

Quality Level

200

form

tablet

mp

258 °C (dec.) (lit.)

solubility

water: 1 tablet/10 mL, clear, colorless

storage temp.

2-8°C

SMILES string

Cl[H].Cl[H].Nc1ccccc1N

InChI

1S/C6H8N2.2ClH/c7-5-3-1-2-4-6(5)8;;/h1-4H,7-8H2;2*1H

InChI key

RIIWUGSYXOBDMC-UHFFFAOYSA-N

¿Está buscando productos similares? Visita Guía de comparación de productos

General description

El diclorhidrato de o-fenilendiamina (OPD) es un sustrato cromógeno que puede utilizarse en procedimientos de enzimoinmunoanálisis de absorción (ELISA), ya que utiliza conjugados de peroxidasa de rábano picante. El sustrato rinde un producto final soluble que es de color naranja-marrón y puede leerse en el espectrofotómetro a 450 nm. La reacción del OPD puede detenerse con HCl 3N o H2SO4 3 M y leerse a 492 nm.

Application

o-Phenylenediamine dihydrochloride has been used:
  • as a substrate in conventional capture enzyme-linked immunosorbent assay (ELISA) for recombinant hemagglutinin (rHA) protein detection
  • as a substrate for the determination of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) immunoglobulin G (IgG) antibodies by ELISA
  • as a colorimetric reagent specific for horseradish peroxidase (HRP) activity assessment in an ELISA protocol using SARS-CoV-2 antigens for S protein

Reconstitution

Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 μl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. Tablets are individually packaged in foil packets.

signalword

Warning

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Irrit. 2 - Muta. 2 - Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

B Abomoelak et al.
Infection and immunity, 67(10), 5100-5105 (1999-09-25)
The development of combined vaccines constitutes one of the priorities in modern vaccine research. One of the most successful combined vaccines in use is the diphtheria-pertussis-tetanus vaccine. However, concerns about the safety of the pertussis arm have led to decreased
Hepatitis B virus transgenic severe combined immunodeficient mouse model of acute and chronic liver disease.
Mark A Feitelson et al.
Methods in molecular medicine, 96, 269-287 (2004-02-06)
Giorgia Squeri et al.
Molecular therapy : the journal of the American Society of Gene Therapy, 27(7), 1215-1227 (2019-05-08)
Mucopolysaccharidosis type I (MPS-I) is a severe genetic disease caused by a deficiency of the alpha-L-iduronidase (IDUA) enzyme. Ex vivo hematopoietic stem cell (HSC) gene therapy is a promising therapeutic approach for MPS-I, as demonstrated by preclinical studies performed in naive
Cassandra Koole et al.
The Journal of pharmacology and experimental therapeutics, 353(1), 52-63 (2015-01-30)
The glucagon-like peptide-1 receptor (GLP-1R) is a class B G protein-coupled receptor that has a critical role in the regulation of glucose homeostasis, principally through the regulation of insulin secretion. The receptor system is highly complex, able to be activated
Florian Krammer et al.
Clinical and vaccine immunology : CVI, 21(8), 1153-1163 (2014-06-20)
Emerging H7N9 influenza virus infections in Asia have once more spurred the development of effective prepandemic H7 vaccines. However, many vaccines based on avian influenza viruses--including H7--are poorly immunogenic, as measured by traditional correlates of protection. Here we reevaluated sera
Ver todos los artículos relacionados

Artículos

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico