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MAK562

Sigma-Aldrich

Branched Chain Amino Acid Kit

sufficient for 100 colorimetric tests

Sinónimos:

BCAA Assay Kit, BCAA Kit

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About This Item

UNSPSC Code:
41116104
NACRES:
NA.28

usage

sufficient for 100 colorimetric tests

Quality Level

100

detection method

colorimetric

relevant disease(s)

endocrinological disorders, diabetes; neurological disorders; gastrointestinal diseases

storage temp.

−20°C

Categorías relacionadas

General description

Leucine, isoleucine and valine, known collectively as branched-chain amino acids (BCAA) are unique amongst the essential amino acids in that they undergo oxidation to a greater extent in the peripheral tissues than in liver1. Concentrations of these amino acids in plasma are altered by moderate and exhausting exercise, by diet and the nutritional status of the individual.2

In this assay, BCAA concentration is determined using a coupled enzyme reaction to catalyze the conversion of a BCAA molecule to its ketone form, which results in the conversion of NAD+ to NADH.3 NADH is detected by a colorimetric indicator, with an absorption maximum of 450 nm, proportional to the amount of BCAA present.

Application

BCAA Kit has been used to quantify BCAA in various types of samples, such as food, dietary supplements, blood, serum and cells.

Features and Benefits

Highly sensitive, simple and high-throughput assay for measuring BCAA concentration.
Detection time: only 30 minutes
Supportive calculator will calculate the reagents needed according to your experimental needs, and analyze your results based on your experimental data.

Click here to download a calculator excel file

Suitability

Suitable for measuring Branched Chain Amino Acids (BCAA′s) in a variety of biological and organic samples.

pictograms

Corrosion
GHS05

signalword

Danger

hcodes

H314

Hazard Classifications

Eye Dam. 1 - Skin Corr. 1B

Storage Class

8A - Combustible corrosive hazardous materials

wgk_germany

WGK 3

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Certificados de análisis (COA)

Lot/Batch Number

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