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Merck

MAK452

Sigma-Aldrich

Glucose-6-Phosphate Dehydrogenase Inhibitor Screening Kit

Sufficient for 100 colorimetric tests

Sinónimos:

G6PDH Inhibitor Screening Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.54

Quality Level

detection method

colorimetric

relevant disease(s)

hematological disorder, anemia; cancer

storage temp.

−20°C

General description

Glucose-6-Phosphate Dehydrogenase (G6PDH) is a cytosolic enzyme in the pentose phosphate pathway which supplies reducing energy to cells by maintaining the level of the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH). G6PDH reduces nicotinamide adenine dinucleotide phosphate (NADP) to NADPH while oxidizing glucose-6-phosphate (G6P). Humans with a genetic deficiency of G6PDH are predisposed to non-immune hemolytic anemia. Recently, studies have found G6PDH plays a critical role in survival, proliferation, and metastasis of cancer cells. Therefore, inhibitors of the enzyme are attractive candidates for new cancer therapeutics.

Application

The Glucose-6-Phosphate Dehydrogenase Inhibitor Screening Kit may be used for:
  • Cancer Research
  • Drug Discovery

Features and Benefits

  • Efficient Inhibitor Screening: Our kit allows for efficient screening of G6PDH inhibitors using just 5 μL of test compound, minimizing effort while ensuring accurate results.
  • Compatibility with High-Throughput Systems: Seamlessly integrating into high-throughput handling systems, our kit guarantees efficiency and accuracy, streamlining your screening processes.

Suitability

The kit is suitable for high throughput inhibitor screening and evaluation of glucose-6-phosphate dehydrogenase inhibitors.

Principle

The Glucose-6-Phosphate Dehydrogenase Inhibitor Screening Kit is based on the reduction of the tetrazolium salt MTT in a NADPH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at A565 nm. The increase in absorbance at A565 nm is proportional to the enzyme activity. The percent inhibition of a test compound can be determined by comparing the activity of G6PDH treated with a test compound to the activity of untreated G6PDH.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

pictograms

Health hazard

signalword

Warning

hcodes

Hazard Classifications

Muta. 2

Storage Class

12 - Non Combustible Liquids


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