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MAK197

Sigma-Aldrich

Succinate Dehydrogenase Assay Kit

Sufficient for 100 Colorimetric tests

Sinónimos:

SDH Activity Assay Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

detection method

colorimetric

relevant disease(s)

neurological disorders

storage temp.

−20°C

Gene Information

human ... SDHA(6389) , SDHAF1(644096) , SDHAF2(54949) , SDHB(6390) , SDHC(6391) , SDHD(6392)
mouse ... SDHA(66945) , SDHAF1(68332) , SDHAF2(66072) , SDHB(67680) , SDHC(66052) , SDHD(66925)
rat ... SDHA(157074) , SDHAF2(361726) , SDHB(298596) , SDHC(289217) , SDHD(363061)

General description

Succinate dehydrogenase (SDH; EC 1.3.5.1) is a mitochondrial enzyme that catalyzes the oxidation of succinate to fumarate and carries electrons from FADH to CoQ in eukaryotes and bacteria. It has a central function in the maintenance of cellular energy metabolism via the Krebs (tricarboxylic acid) cycle and the electron transport chain. Mutations in SDH cause hereditary paraganglioma/phaeochromocytoma syndrome and a neurodegenerative disorder known as Leigh syndrome.

Application

Succinate Dehydrogenase Activity Colorimetric Assay Kit has been used to measure the enzyme activity of succinate dehydrogenase in samples.

Suitability

Suitable for the detection of Succinate Dehydrogenase Activity in a variety of biological samples such as cell and tissue culture supernatants and purified mitochondria.

Principle

The Succinate Dehydrogenase Activity Colorimetric Assay kit provides a simple and sensitive procedure for measuring SDH activity in a variety of tissues, cells, and isolated mitochondria. SDH activity is determined by generating a product with absorbance at 600 nm proportional to the enzymatic activity present. One unit of SDH is the amount of enzyme that generates 1.0 μmole of DCIP per minute at pH 7.2 at 25 °C.

Solo componentes del kit

Referencia del producto
Descripción
  • SDH Assay Buffer
  • SDH Substrate Mix
  • SDH Probe
  • DCIP Standard, 2 mM
  • SDH Positive Control

Replaced by

Referencia del producto
Descripción
Precios

Storage Class

10 - Combustible liquids

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Certificados de análisis (COA)

Lot/Batch Number
8A14K06600
7C11K06600
6G23K06600
6B06K06600
B1C100660V

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