Saltar al contenido
Merck
Todas las fotos(2)

Documentos clave

Ver Toda la documentación

MAK070

Sigma-Aldrich

Oxaloacetate Assay Kit

sufficient for 100 colorimetric or fluorometric tests

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
Todas las fotos(2)

About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 100 colorimetric or fluorometric tests

detection method

colorimetric
fluorometric

relevant disease(s)

cancer

storage temp.

−20°C

General description

Oxaloacetate (OAA) is an intermediate of both the TCA cycle and gluconeogenesis. It precedes citrate which is formed by the citrate synthase-mediated condensation of acetyl-CoA and OAA. OAA is also formed by the deamidation of aspartate or condensation of CO2 with pyruvate or PEP. Since mammals do not possess the enzymatic machinery to form TCA cycle intermediates from acetyl-CoA, OAA is one of the anaplerotic entry points via pyruvate and pyruvate carboxykinase. The Oxaloacetate Assay Kit provides a simple, sensitive and rapid means of quantifying OAA in a variety of samples.

Application

Oxaloacetate Assay Kit has been used to determine the concentration of oxaloacetate in samples.

Suitability

Suitable for animal tissues, cell culture (adherent or suspension cells), serum and saliva.

Principle

In this kit, oxaloacetate concentration is determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/fluorometric (lex = 535/lem = 587 nm) product, proportional to the oxaloacetate present. Typical detection ranges for this kit are 2–10 nmole (colorimetric) and 0.2–1 nmole (fluorometric).

Replaced by

Referencia del producto
Descripción
Precios

pictograms

Health hazard
GHS08

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Sens. 1

Storage Class

10 - Combustible liquids

Elija entre una de las versiones más recientes:

Certificados de análisis (COA)

Lot/Batch Number
6J11K06590
5G15K06590
5A16K06590
4G20K06590
3C24K06590

¿No ve la versión correcta?

Si necesita una versión concreta, puede buscar un certificado específico por el número de lote.

Busque por un COA

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

The transcription factor Cabut coordinates energy metabolism and the circadian clock in response to sugar sensing.
Bartok O, Teesalu M, Ashwall-Fluss R, et al.
The Embo Journal, 34(11), 1538-1553 (2015)
Zheng Li et al.
Stem cells (Dayton, Ohio), 37(12), 1542-1555 (2019-10-02)
Mitochondrial phosphoenolpyruvate carboxykinase (PCK2) is a rate-limiting enzyme that plays critical roles in multiple physiological processes. The decompensation of PCK2 leads to various energy metabolic disorders. However, little is known regarding the effects of PCK2 on osteogenesis by human mesenchymal
Huabo Wang et al.
The Journal of biological chemistry, 294(14), 5466-5486 (2019-02-14)
Eukaryotic cell metabolism consists of processes that generate available energy, such as glycolysis, the tricarboxylic acid (TCA) cycle, and oxidative phosphorylation (Oxphos), and those that consume it, including macromolecular synthesis, the maintenance of ionic gradients, and cellular detoxification. By converting
Ke Tang et al.
Oncogene, 38(44), 6970-6984 (2019-08-15)
Clinical applications of antiangiogenic agents profoundly affect tumor cell behaviors via the resultant hypoxia. To date, how the hypoxia regulates tumor cells remains unclear. Here, we show that hypoxia promotes the growth of human breast tumorigenic cells that repopulate tumors
The transcription factor Cabut coordinates energy metabolism and the circadian clock in response to sugar sensing.
Bartok O, et al.
The Embo Journal, 34(11), 1538-1553 (2015)
Ver todos los artículos relacionados

Artículos

Glutamine Metabolism is Dysregulated in Many Cancer Cells

Sigma-Aldrich presents an article about how proliferatively active cells require both a source of carbon and of nitrogen for the synthesis of macromolecules. Although a large proportion of tumor cells utilize aerobic glycolysis and shunt metabolites away from mitochondrial oxidative phosphorylation, many tumor cells exhibit increased mitochondrial activity.

Fatty Acid Synthesis and Metabolism in Cancer Cells

Information on fatty acid synthesis and metabolism in cancer cells. Learn how proliferatively active cells require fatty acids for functions such as membrane generation, protein modification, and bioenergetic requirements. These fatty acids are derived either from dietary sources or are synthesized by the cell.

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico