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M4530

Sigma-Aldrich

Medium 199

With Earle′s salts, ʟ-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Sinónimos:

M199 Medium, TCM 199

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

product name

Medium 199, With Earle′s salts, L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Quality Level

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

NaHCO3: yes
L-glutamine: yes
HEPES: no
Earle’s salts (5% CO2): yes
phenol red: yes
sodium pyruvate: no

shipped in

ambient

storage temp.

2-8°C

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General description

Complete liquid version of the original M 199 formulation published by Morgan.
Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.

Application

Medium 199 has been used:
  • as a suspension media to maintain the primary neuronal cultures
  • as a component of the collagen gel for ex vivo embryonic heart culture
  • to maintain the ovarian tissue sections as part of the slow-freezing protocol

also commonly purchased with this product

Referencia del producto
Descripción
Precios

supplement

Referencia del producto
Descripción
Precios

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Patrick C Baer et al.
International journal of molecular sciences, 19(6) (2018-06-13)
Stem cell-based therapies require cells with a maximum regenerative capacity in order to support regeneration after tissue injury and organ failure. Optimization of this regenerative potential of mesenchymal stromal/stem cells (MSC) or their conditioned medium by in vitro preconditioning regimens
Li Ma et al.
The journals of gerontology. Series A, Biological sciences and medical sciences, 72(4), 489-498 (2016-08-28)
We previously reported that pretreatment with the potent antioxidant TEMPOL improves mitochondrial function and restores preconditioning in the aging heart. Because mitophagy is implicated in cardiac preconditioning and declines with age, this study was designed to investigate how age influences
Vladimir Jovasevic et al.
Journal of virology, 82(7), 3311-3319 (2008-01-25)
In this report we propose a model in which after the herpes simplex virus (HSV) capsid docks at the nuclear pore, the tegument protein attached to the capsid must be cleaved by a serine or a cysteine protease in order
Amanda Fanous et al.
Plastic and reconstructive surgery, 143(3), 760-767 (2019-01-03)
Keloid scarring is a serious condition that mostly affects patients of African or Asian descent. Often disfiguring, this condition can have devastating psychosocial consequences. To date, no treatment modality has been proven ideal. The authors' objectives were (1) to determine
Immacolata Castellano et al.
Oxidative medicine and cellular longevity, 2018, 2087373-2087373 (2018-06-01)
Chronic hyperglycemia is associated with oxidative stress and vascular inflammation, both leading to endothelial dysfunction and cardiovascular disease that can be weakened by antioxidant/anti-inflammatory molecules in both healthy and diabetic subjects. Among natural molecules, ovothiol A, produced in sea urchin
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Medium 199 Formulation

Medium 199 Formulation

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