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Merck

M0535

Sigma-Aldrich

Mycoplasma Broth

powder, suitable for microbiology

Sinónimos:

Mycoplasma media

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About This Item

UNSPSC Code:
41106200
NACRES:
NA.74

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form

powder

Quality Level

technique(s)

microbiological culture: suitable

solubility

H2O: 25.5 g/L (Autoclave at 121°C, 15 psi for 15 minutes. Store prepared medium at 0-5°C.)

application(s)

microbiology

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M0660M0178T9179
Terrific Broth EZMix powder microbial growth medium

T9179

Terrific Broth

technique(s)

microbiological culture: suitable

technique(s)

-

technique(s)

microbe id | susceptibility testing: suitable, microbiological culture: suitable

technique(s)

microbiological culture: suitable

application(s)

microbiology

application(s)

microbiology

application(s)

clinical testing
food and beverages
life science and biopharma
veterinary, microbiology

application(s)

agriculture
food and beverages
microbiology

solubility

H2O: 25.5 g/L (Autoclave at 121°C, 15 psi for 15 minutes. Store prepared medium at 0-5°C.)

solubility

H2O: 35.5 g/L

solubility

-

solubility

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

Mycoplasma propagation

Mycoplasma propagation

-

-

General description

Mycoplasma Broth is a basal medium which, after enrichment with yeast extract, horse serum, and antibiotics, will support the growth of Mycoplasma sp. The components contained in this basal medium do not exhibit inhibitory or toxic effects when used to culture mycoplasma. A special mineral supplement has been added to improve the growth and colony characteristics of the organism.

Application

Mycoplasma Broth has been used for isolation and cultivation of mycoplasma.[1] It may also be used to grow Porphyromonas gingivalis under anerobic conditions.[2]

Components

Powder contains (g/L): 10.0 bacterial peptone, 10.0 LAB-LEMCO powder, 5.0 sodium chloride, 0.5 mineral supplement.

Preparation Note

Basal Medium:

1. Suspend 25.5 g of powdered medium in 1L of distilled water. Stir until the powder dissolves.

2. Adjust the pH of the solution to 7.8 ± 0.2.

3. Autoclave at 121 °C and 15 psi for 15 minutes to sterilize. Cool to 50 °C and add sterile supplements.

Storage and Stability

Store dry powdered medium at room temperature and prepare medium at 2-8 °C.

Other Notes

The product is intended for laboratory use only.

Optional

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Philip Stashenko et al.
The American journal of pathology, 170(1), 203-213 (2007-01-04)
Bacterial infections of the dental pulp result in soft tissue and alveolar bone destruction. It has been suggested that Th1 responses promote disease, whereas Th2 responses are protective. However, other studies have challenged this notion. To address this question, bone
Davor Ivankovic et al.
Autophagy, 16(3), 391-407 (2019-05-31)
Adaptor protein (AP) complexes mediate key sorting decisions in the cell through selective incorporation of transmembrane proteins into vesicles. Little is known of the roles of AP-4, despite its loss of function leading to a severe early onset neurological disorder

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