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L9420

Sigma-Aldrich

Luciferase from Photinus pyralis (firefly)

recombinant, expressed in E. coli, buffered aqueous solution, ≥10×1010 units/mg protein

Sinónimos:

Luciferase firefly

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About This Item

Número de CAS:
61970-00-1
Comisión internacional de enzimas:
1.13.12.7 (BRENDA, IUBMB)
MDL number:
MFCD00131546
UNSPSC Code:
12352204
NACRES:
NA.54

recombinant

expressed in E. coli

Quality Level

200

assay

≥98% (SDS-PAGE)

form

buffered aqueous solution

specific activity

≥10×1010 units/mg protein

mol wt

62 kDa

shipped in

dry ice

storage temp.

−20°C

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General description

Firefly luciferase catalyzes the reaction of luciferin with ATP and leads to the production of yellow-green light. The enzyme has a molecular weight of 62kDa and is expressed in peroxisomes. Luciferase is considered as a model to study protein–anesthetic interactions. Firefly luciferase is highly useful in cell biology and molecular biology, as a reporter of gene function and for the quantification of ATP.

Application

Luciferase from Photinus pyralis (firefly) has been used as a component of lysis solution to measure luminescence signals, as part of the study to determine chemical signals which can activate the karrikin insensitive 2 (KAI2) pathway.

Unit Definition

One luciferase enzyme unit will produce one Relative Light Unit (RLU) at 20-25 °C over a 10 second period, measured in 100 μl assay mixture containing 40 pmol ATP and 15 nmol luciferin in Tris-glycine buffer, pH 7.6, using a GloMax 20/20 Luminometer.

Unit Definition Conversion Factor: There are approximately 9000 Relative Light Units (RLU) per one traditional Light Unit that uses a peak height equivalent to 0.02 μCi of 14C in a PPO/POPOP cocktail.

Physical form

Supplied a a solution in 25mM Tris-acetate, pH 7.8 , 1mM EDTA, 1mM DTT, 0.2M ammonium sulfate, 15% glycerol and 30% ethylene glycol

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Resp. Sens. 1 - STOT RE 2 Oral

target_organs

Kidney

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

231.8 °F

flash_point_c

111 °C

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Certificados de análisis (COA)

Lot/Batch Number
11190522
128M4087V
11180738
058M4116V
11171216

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Griffiths A J F, et al.
An Introduction to Genetic Analysis. (2000)
Crystal structure of firefly luciferase throws light on a superfamily of adenylate-forming enzymes.
Conti E, et al.
Structure, 4(3), 287-298 (1996)
Reporter gene-facilitated detection of compounds in Arabidopsis leaf extracts that activate the Karrikin signaling pathway.
Sun Y K, et al.
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Methods in molecular biology (Clifton, N.J.), 1087, 299-313 (2013-10-26)
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Methods in molecular biology (Clifton, N.J.), 1098, 21-32 (2013-10-30)
Adenosine 5'-triphosphate (ATP) is the "energy currency" of organisms and plays central roles in bioenergetics, whereby its level is used to evaluate cell viability, proliferation, death, and energy transmission. In this chapter, we describe an improved and efficient method for
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