L6136
Lipopolysaccharides from Serratia marcescens
purified by phenol extraction
Sinónimos:
LPS
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About This Item
Productos recomendados
biological source
Serratia marcescens
Quality Level
form
lyophilized powder
purified by
phenol extraction
impurities
<3% Protein (Lowry)
color
white to yellow cast
solubility
water: 4.90-5.10, faintly hazy to hazy, colorless to faint yellow or tan
shipped in
ambient
storage temp.
2-8°C
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General description
This product is phenol extracted from Serratia marcescens. The source strain is ATCC 21639.
Biochem/physiol Actions
Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.
Preparation Note
The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.
Other Notes
To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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H Heremans et al.
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Journal of biomedical science, 19, 69-69 (2012-08-02)
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