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H8889

Sigma-Aldrich

Histopaque®-1077 Hybri-Max™

liquid, sterile-filtered, BioReagent, suitable for hybridoma

Sinónimos:

Histopaque 1077

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About This Item

UNSPSC Code:
12352201
NACRES:
NA.75

sterility

sterile-filtered

Quality Level

400

product line

BioReagent

form

liquid

technique(s)

cell culture | hybridoma: suitable
single cell analysis: suitable

impurities

endotoxin, tested

density

1.077 at 25 °C (relative density)(lit.)

storage temp.

2-8°C

General description

Histopaque®-1077 is a solution comprising polysucrose and sodium diatrizoate, adjusted to a density of 1.077 +/- 0.001 g/cm3. This medium facilitates rapid recovery of viable lymphocytes from small volumes of whole blood. This technique is suitable for use in cell-mediated lympholysis and human lymphocyte antigen (HLA)2 typing. It may also be employed as the initial isolation step before the enumeration of T-, B-, and null lymphocytes. It is used to create a density medium for the purification of lymphocytes and other mononuclear cells.

Application

Histopaque®-1077 Hybri-Max™ has been used to isolate:
  • peripheral blood mononuclear cells (PBMCs) from heparinized blood by gradient centrifugation
  • immune cells (lymphocytes) from peripheral blood
  • PBMCs from blood

Legal Information

Histopaque is a registered trademark of Merck KGaA, Darmstadt, Germany
Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

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Referencia del producto
Descripción
Precios

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Q Liu et al.
Luminescence : the journal of biological and chemical luminescence, 14(6), 327-330 (1999-12-22)
Decaglycerol mono-oleate (DGMO), a type of food additive (a polyglycerol ester of fatty acids), was evaluated for its in vitro effect on reactive oxygen species (ROS) generation by human neutrophils using a luminol-dependent chemiluminescence assay. Neutrophils were isolated by the
Peter R C Gascoyne et al.
Electrophoresis, 30(8), 1388-1398 (2009-03-24)
The application of dielectrophoretic field-flow fractionation (depFFF) to the isolation of circulating tumor cells (CTCs) from clinical blood specimens was studied using simulated cell mixtures of three different cultured tumor cell types with peripheral blood. The depFFF method can not
Joshua E Messinger et al.
mBio, 10(4) (2019-07-04)
Deciphering the molecular pathogenesis of virally induced cancers is challenging due, in part, to the heterogeneity of both viral gene expression and host gene expression. Epstein-Barr virus (EBV) is a ubiquitous herpesvirus prevalent in B-cell lymphomas of immune-suppressed individuals. EBV
Siddharth Sharma et al.
Cytometry. Part A : the journal of the International Society for Analytical Cytology, 73(2), 160-167 (2008-01-15)
Coulter volume is far more accurate measure of cell volume than forward angle light scatter. In this report, we have used Coulter volume to determine the mean cell volume and diameter of normal human peripheral blood cells and hematopoietic progenitor
Janet E Alexander et al.
The journals of gerontology. Series A, Biological sciences and medical sciences, 73(6), 720-728 (2017-11-11)
As in human populations, advances in nutrition and veterinary care have led to an increase in the lifespan of companion animals. Detrimental physiological changes occurring later in life must be understood before interventions can be made to slow or reduce
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